Search of potential non-ribosomal peptide producers (NRPS) in the compost from the foundation Parque Zoológico de São Paulo using iChip and Prism

• Grant number: 17/08038-8
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: June 01, 2017
• End date: May 31, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Luiz Juliano Neto
• Grantee: Luciana Teresa Dias Cappelini
• Supervisor: Neil Kelleher
• Host Institution: Instituto Nacional de Farmacologia (INFAR). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Institution abroad: Northwestern University, Evanston, United States
• Associated to the scholarship: 14/07037-0 - Chemical and biological assessment of composting produced at the Fundação Parque Zoológico de São Paulo (FPZSP)., BP.PD

Abstract

Alternatives for the disposing of organic waste generated by our society are always a necessity. Among this alternatives the Fundação Parque Zoológico de São Paulo (FPZSP) uses organic waste generated daily in the park for production of composting, which is subsequently used inside of the ZOO and also its farm (Unidade de Produção Rural -UPR) where it is produced most of the food consumed by the animals. Composting is the result of digestion of organic matter by the action of macro and mesofauna and of different communities of microorganisms, and this result supplies the plants with absorbable forms of nutrients that they need. Besides composting, there is also the generation of leachate that is generated from the water to cool off the composters; This material is still little exploited in the FPZSP. With the aim of searching for new alternatives for composting, the objective of this stage is to learn the techniques of identification of non-ribosomal peptides using genomics, proteomics and metabolomics together with the use of LC-MS/MS, already based in Professor Kelleher 's laboratory. The training will allow molecules of interest, such as antibiotics, to be sought in FPZSP's composting and leachate. For this, microorganisms from these matrices will be isolated using iChip, device that allows the cultivation of organisms not yet identified in their own habitat. The iChip has already been applied in FPZSP leachate and has shown promise in isolating microorganisms.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NAVARRO-MUNOZ, JORGE C.; SELEM-MOJICA, NELLY; MULLOWNEY, MICHAEL W.; KAUTSAR, SATRIA A.; TRYON, JAMES H.; PARKINSON, I, ELIZABETH; DE LOS SANTOS, EMMANUEL L. C.; YEONG, MARLEY; CRUZ-MORALES, PABLO; ABUBUCKER, SAHAR; et al. A computational framework to explore large-scale biosynthetic diversity. Nature Chemical Biology, v. 16, n. 1, p. 60+, JAN 2020. (17/08038-8)

BELINATO, JOAO RAUL; ACQUARO, JR., VINICIUS RICARDO; DIAS CAPPELINI, LUCIANA TERESA; AUGUSTO, FABIO. New prospects and problems in sample preparation methods for microbiome analysis. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 143, OCT 2021. (18/25729-7, 17/08038-8, 18/03956-1, 16/20547-2, 14/07037-0)