Degradation and in situ ruminal microbial dynamics of different sources of protein for beef cattle

• Grant number: 17/02034-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2017
• End date: August 31, 2020
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: Telma Teresinha Berchielli
• Grantee: Yury Tatiana Granja Salcedo
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The objective of this project is to evaluate the ruminal microbial diversity involved in the ruminal degradation of soybean meal, cottonseed meal, DGGS and glutenose, and correlate it with ruminal degradation of DM, CP and protein fractions of each ingredient. In each experimental period, after 20 days of adaptation of the animals to the diet, 112 nylon bags in situ containing 5 g of each protein source will be placed in the rumen of cannulated steers fed with the respective protein source. Four nylon bags will be placed in the animals' rumen in reverse chronological order at 48, 24,16, 6, 4, 2 e 0.5h before being removed and eight non-incubated nylon bags filled with the different ingredients will be used as control (0 h). The relative degradation of the biomass during rumen incubation will be determined by MS analysis according to the AOAC methods and CP by multiplication by 6.25 of the nitrogen determined in the LECO FP-528 nitrogen analyzer. CP insoluble in neutral detergent, CP insoluble acid detergent, non-protein nitrogen and soluble protein content of protein sources will be estimated according to Licitra et al. (1996). The nylon bags recovered from the rumen for microbiological analyzes will be washed with PBS buffer solution and immediately frozen and storage in liquid nitrogen and transported to the laboratory for the extraction of metagenomic DNA. Sequencing of the V4/V5 region of the bacterial 16S rRNA gene will be the method used for the identification and quantification of adhered ruminal bacteria. The results will be analyzed considering a 4 x 4 Latin square design, with measures repeated over time.

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMEZ-INSUASTI, ARTURO SAMUEL; GRANJA-SALCEDO, YURY TATIANA; CASTILLO VARGAS, JULIAN ANDRES; MESSANA, JULIANA DUARTE; DE OLIVEIRA SADER, ANA PAULA; BERCHIELLI, TELMA TERESINHA. In vitro gas production and fatty acids biohydrogenation of diets containing different unsaturated fatty acids sources plus crude glycerin. TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, v. 45, n. 5, p. 944-954, 2021. (17/02034-0, 12/23949-3)

ERICK ESCOBAR DALLANTONIA; LORRAYNY GALORO DA SILVA; YURY TATIANA GRANJA-SALCEDO; JULIANA DUARTE MESSANA; LIZIANE DE FIGUEIREDO BRITO; ANA REBECA CASTRO LIMA; ELIAS SAN VITO; PABLO DE SOUZA CASTAGNINO; AMÉRICO GARCIA DA SILVA SOBRINHO; RICARDO ANDRADE REIS; et al. Association of additives in supplemented grazing cattle during the finishing phase at the rainy season. REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE, v. 52, 2023-03-03. (17/02034-0, 15/05216-7, 15/01147-0, 19/12740-5)

GOMES CARVALHO ALVES, KENIA LARISSA; GRANJA-SALCEDO, YURY TATIANA; MESSANA, JULIANA DUARTE; DE SOUZA, VINICIUS CARNEIRO; GENEROSO GANGA, MARIA JULIA; DETOGNI COLOVATE, PAULO HENRIQUE; KISHI, LUCIANO TAKESHI; BERCHIELLI, TELMA TERESINHA. Rumen bacterial diversity in relation to nitrogen retention in beef cattle. Anaerobe, v. 67, FEB 2021. (17/02034-0, 16/16796-7, 18/13885-4, 16/22022-4, 17/11571-0)

CAMPOS, LETICIA M.; SOUZA, VINICIUS C.; GRANJA-SALCEDO, YURY T.; MESSANA, JULIANA D.; PRESTEGAARD-WILSON, JACQUELYN M.; GANGA, MARIA JULIA G.; DIAS, ANA VERONICA L.; COSTA, VLADIMIR E.; BERCHIELLI, TELMA T.. Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources. JOURNAL OF ANIMAL SCIENCE, v. 100, n. 5, p. 11-pg., 2022-05-01. (16/22022-4, 17/02034-0, 17/08854-0, 16/16796-7)

GRANJA-SALCEDO, YURY TATIANA; FERNANDES, RODOLFO MACIEL; DE ARAUJO, RAFAEL CANONENCO; KISHI, LUCIANO TAKESHI; BERCHIELLI, TELMA TERESINHA; DE RESENDE, FLAVIO DUTRA; BERNDT, ALEXANDRE; SIQUEIRA, GUSTAVO REZENDE. Long-Term Encapsulated Nitrate Supplementation Modulates Rumen Microbial Diversity and Rumen Fermentation to Reduce Methane Emission in Grazing Steers. FRONTIERS IN MICROBIOLOGY, v. 10, MAR 29 2019. (17/02034-0)

SIMIONI, TIAGO A.; TORRECILHAS, JULIANA A.; MESSANA, JULIANA D.; GRANJA-SALCEDO, YURY T.; SAN VITO, ELIAS; LIMA, ANA R. C.; SANCHEZ, JOAO M. D.; REIS, RICARDO A.; BERCHIELLI, TELMA T.. Influence of growing-phase supplementation strategies on intake and performance of different beef cattle genotypes in finishing phase on pasture or feedlot. LIVESTOCK SCIENCE, v. 251, SEP 2021. (15/01147-0, 17/02034-0)

DIAS, ANA VERONICA LINO; MESSANA, JULIANA DUARTE; GRANJA-SALCEDO, YURY TATIANA; ALFONSO, YEISON FABIAN MURILO; SILVA, LORRAYNY GALORO; CAMARGO, KARINE DALLA VECCHIA; ALVES, KENIA LARISSA GOMES CARVALHO; GONCALVES, PALOMA HELENA; REIS, RICARDO ANDRADE; BERCHIELLI, TELMA TERESINHA. Efficiency of Amino Acid Utilization in Nellore Cattle Grazing Low-Quality Forage Supplemented with Different Sources of Nitrogen. LIFE-BASEL, v. 13, n. 8, p. 15-pg., 2023-08-01. (18/06836-7, 19/21280-8, 16/22022-4, 19/12740-5, 15/01147-0, 17/02034-0)

GRANJA-SALCEDO, YURY TATIANA; FERNANDES, RODOLFO MACIEL; DE ARAUJO, RAFAEL CANONENCO; KISHI, LUCIANO TAKESHI; BERCHIELLI, TELMA TERESINHA; DE RESENDE, FLAVIO DUTRA; BERNDT, ALEXANDRE; SIQUEIRA, GUSTAVO REZENDE. Long-Term Encapsulated Nitrate Supplementation Modulates Rumen Microbial Diversity and Rumen Fermentation to Reduce Methane Emission in Grazing Steers (vol 10, 674, 2019). FRONTIERS IN MICROBIOLOGY, v. 10, p. 1-pg., 2019-07-29. (17/02034-0)