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The role of pyruvate carboxylase on insulin sensitivity of skeletal muscle cells line of mice submitted to aminoacids deprivation: Involviment of ACILCARNITINES

Grant number: 16/24289-8
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): July 01, 2017
Effective date (End): August 31, 2021
Field of knowledge:Biological Sciences - Biochemistry - Metabolism and Bioenergetics
Principal Investigator:Everardo Magalhães Carneiro
Grantee:Renato Chaves Souto Branco
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:13/07607-8 - OCRC - Obesity and Comorbidities Research Center, AP.CEPID

Abstract

Lipid overload to skeletal muscle has been associated to increase in fatty acid oxidation and generation of acylcarnitines (AC) and insulin resistance (IR). This may happen due the inefficiency of TCA in oxidize the Acetyl-CoA produced by fatty acid oxidation process. Interventions that blunt the LCAcyl-CoA transportation into the mitochondria reduce the generation of AC as well as improve muscular IR induced by high-fat diet (HFD). However, strategies focusing the enhancement of TCA function, in order to prevent the AC generation and IR onset, have not yet been investigated. The expression of the anaplerotic enzyme, pyruvate carboxylase (PYC), is reduced in skeletal muscle of HFD fed mice, which accumulates high amount of intramuscular (IM) triacylglycerol (TAG), and are insulin resistant. On the other hand, mice fed a low protein diet also accumulates IM TAG, nevertheless, display increased muscle insulin sensitivity and PYC protein content. This data suggest that PYC may be important for muscular IR onset. Our goal is to investigate a possible involvement of PYC on MIR onset, as well as, whether its activity is correlated to the phenotype found in protein restricted mice skeletal muscle. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOUTO BRANCO, RENATO CHAVES; CAMARGO, RAFAEL LUDEMANN; BATISTA, THIAGO MARTINS; VETTORAZZI, JEAN FRANCIESCO; LUBACZEUSKI, CAMILA; MONTES BOMFIM, LUCAS HENRIQUE; SILVEIRA, LEONARDO REIS; BOSCHERO, ANTONIO CARLOS; ZOPPI, CLAUDIO CESAR; CARNEIRO, EVERARDO MAGALHAES. Protein malnutrition mitigates the effects of a high-fat diet on glucose homeostasis in mice. Journal of Cellular Physiology, v. 234, n. 5, p. 6313-6323, MAY 2019. Web of Science Citations: 0.

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