Immunomodulatory effect of probiotic bacteria: analysis in peripheral monocytes and dendritic cells.

• Grant number: 17/10829-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2017
• End date: January 31, 2019
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Marcia Pinto Alves Mayer
• Grantee: Gláuber Campos Vale
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 15/18273-9 - New strategies for the control of periodontitis, AP.TEM

Abstract

This proposal aims to establish fundamentals for the development of new strategies for periodontitis control. The general hypothesis to be tested is that the equilibrium in oral microbial-immune response relation, lost in periodontitis, could be recovered by favoring colonization of oral cavity by beneficial bacteria and modulation of immune response. The present project aims to determine the ability of probiotic strains to modulate the immune response in vitro and in animal models. Peripheral monocytes and dendritic cells will be used in co-culture assays with periodontal pathogens. The cytokine profile, expression of genes associated with the inflammatory process, activation of signaling pathways and functionality in the differentiation of T lymphocyte subtypes will be evaluated. This strategy aims to select probiotic strain(s) capable of inducing a response that changes the oral and intestinal microbiota favoring the health-associated microbiota, in balance with the host. These results will contribute to the establishment of capable strategies to interfering with the dysbiosis microbiota and host-response to the resident microbiota, favoring the maintenance of periodontal health.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VALE, GLAUBER CAMPOS; MOTA, BRENDA IZABELA SANTANA; ANDO-SUGUIMOTO, ELLEN SAYURI; MAYER, MARCIA PINTO ALVES. Lactobacilli Probiotics Modulate Antibacterial Response Gene Transcription of Dendritic Cells Challenged with LPS. PROBIOTICS AND ANTIMICROBIAL PROTEINS, v. N/A, p. 15-pg., 2023-01-25. (15/18273-9, 17/10829-3)

KAWAMOTO, DIONE; LUCAS AMADO, PAMELA PONTES; ALBUQUERQUE-SOUZA, EMMANUEL; BUENO, MANUELA ROCHA; VALE, GLAUBER CAMPOS; SARAIVA, LUCIANA; ALVES MAYER, MARCIA PINTO. Chemokines and cytokines profile in whole saliva of patients with periodontitis. CYTOKINE, v. 135, p. 9-pg., 2020-11-01. (16/13159-6, 17/16377-7, 15/00259-0, 17/10829-3, 15/18273-9)

VALE, GLAUBER CAMPOS; SANTANA MOTA, BRENDA IZABELA; ANDO-SUGUIMOTO, ELLEN SAYURI; ALVES MAYER, MARCIA PINTO. Effect of Probiotics Lactobacillus acidophilus and Lacticaseibacillus rhamnosus on Antibacterial Response Gene Transcription of Human Peripheral Monocytes. PROBIOTICS AND ANTIMICROBIAL PROTEINS, AUG 2021. (15/18273-9, 17/10829-3)

VALE, GLAUBER CAMPOS; ALVES MAYER, MARCIA PINTO. Effect of probiotic Lactobacillus rhamnosus by-products on gingival epithelial cells challenged with Porphyromonas gingivalis. ARCHIVES OF ORAL BIOLOGY, v. 128, AUG 2021. (17/10829-3, 15/18273-9)