Immunoexpression study of Langerhans cells's markers in oral submucous fibrosis

• Grant number: 17/15633-0
• Support type: Scholarships in Brazil - Master
• Effective date (Start): November 01, 2017
• Effective date (End): February 28, 2019
• Field of knowledge: Health Sciences - Dentistry
• Principal researcher: Pablo Agustin Vargas
• Grantee: Luan César da Silva
• Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Introduction: Oral submucous fibrosis (OSF) is a public health problem that affects the Asian continent, mostly the Indian subcontinent. Its known that its manifestation is intimately correlated with the habit of Bethel chewing, which characterizes the high potential for malignization of these lesions. Immune system cells, such as Langerhans cells (LC) and dendritic cells (DC), are linked to the immunopathogenesis of various lesions and have been studied for their ability to recognize precursor malignant cells and to destroy them. Aim: Investigate the distribution profile of LC and DC in OSF and oral cancer, and to correlate it with the prognosis of these lesions. Material and Methods: This study includes 27 cases of OSF from the Biobank of the Piracicaba Dental School (UNICAMP), 50 cases diagnosed as Oral Squamous Cell Carcinoma (OSCC) collected at the AC Camargo Cancer Center and 10 cases of mucocele from the Orocentro - Piracicaba Dental School (UNICAMP). The cases will be collected with different grades of epithelial dysplasia that will be submitted to immunohistochemical reactions using CD1a, CD207, CD83 and CD303 as molecular markers. The clinical and follow-up data of patients included in the study will be collected through consultation of their respective medical files. The statistical analysis will be composed of association analysis, correlation analysis and analysis of variance. Expected Results: By the end of this research project we look forward to the better understanding of the importance of LC in oral carcinogenesis, as well as its potential as prognostic determinant. In addition to collaborating with the literature, since only two studies have investigated the distribution of LC in OSF. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, LUAN CESAR; FONSECA, FELIPE PAIVA; DE ALMEIDA, OSLEI PAES; LINHARES DE ALMEIDA MARIZ, BRUNO AUGUSTO; LOPES, MARCIO AJUDARTE; RADHAKRISHNAN, RAGHU; SHARMA, MOHIT; KOWALSKI, LUIZ PAULO; VARGAS, PABLO AGUSTIN. CD1a+and CD207+cells are reduced in oral submucous fibrosis and oral squamous cell carcinoma. MEDICINA ORAL PATOLOGIA ORAL Y CIRUGIA BUCAL, v. 25, n. 1, p. E49-E55, JAN 2020. Web of Science Citations: 1.