Imunoexpression of dendritic cells in oral potentially maligant disorders and oral squamous cell carcinoma

Author(s)

Luan César da Silva

• Document type: Master's Dissertation
• Press: Piracicaba, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
• Defense date: 2019-02-25

Examining board members

Pablo Agustin Vargas; Livia Maris Ribeiro Paranaiba; Alan Roger Santos Silva

• Advisor: Felipe Paiva Fonseca; Pablo Agustin Vargas

Abstract

Oral cancer is a public health major problem worldwide. Approximately 90% of oral neoplasms are squamous cell carcinomas (OSCC). OSCC has a multifactorial etiology, varying in the global incidence representing the sixth most common malignancy in the world, but in some Asian countries like Sri Lanka, India, Pakistan, and Bangladesh, it is even more prevalent. In approximately one-third of cases, OSCC can arise from oral potentially malignant disorders (OPMDs), such as oral leukoplakia (OL) and oral submucous fibrosis (OSMF). However, the malignant transformation potential of these OPMDs is difficult to determine only by clinical and microscopic features. Thus, the importance of the immune system in the development and behavior of OPMDs (such as OL and OSMF) and frankly invasive lesions (such as OSCC) has been widely investigated. In this line, the aim of this study was to determine the distribution of immature dendritic cells (DC) and plasmacytoid DCs in OSMF, OSMF associated with OSCC (OSMF-OSCC), OL and OSCC. For this, fourteen cases of OSMF, 9 of OSMF-OSCC, 8 of OL 45, 45 of OSCC and 8 of mucoceles were retrospectively retrieved and had their diagnoses confirmed. Immunoreactions against CD1a, CD207 e CD303 were performed and the number of positive cells quantified. The results showed a significant difference for CD1a+ (p<0.01) and CD207+ (p <0.01) antibodies. A significant decrease was observed in CD1a+ cells in OSMF (p'< or =' 0.05), OSMF-OSCC (p '< or =' 0.01) and OSCC (p '< or =' 0.001) when compared to normal epithelium. For CD207+ the significance was observed in OSMF-OSCC (p '< or =' 0.05), and OSCC (p '< or =' 0.01) when compared with normal epithelium, and in OSMF when compared with OL (p '< or =' 0.05). There was no significant difference in CD303 (p: 0.09). In conclusion, the decrease in the number of CD1a+ and CD207+ cells may be associated with the development of OSCC, and in OPMDs might be an indicator of malignant transformation (AU)

• FAPESP's process: 17/15633-0 - Immunoexpression study of Langerhans cells's markers in oral submucous fibrosis
• Grantee: Luan César da Silva
• Support Opportunities: Scholarships in Brazil - Master