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Dual transcriptome profiling of murine macrophages infected with Leishmania amazonensis

Grant number: 16/03273-6
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2018
Effective date (End): February 28, 2021
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Lucile Maria Floeter-Winter
Grantee:Juliana Ide Aoki
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Leishmania is a protozoan parasite that alternates its life cycle between the sand fly and the mammalian hosts. This alternation involves environmental changes and submits the parasite to dynamic modifications in morphology, metabolism, cellular signaling and gene expression regulation to allow a rapid adaptation to new conditions. Our research group have been studying the role of arginase in L. amazonensis during the parasite life cycle and its role in the establishment and maintenance of the infection in mammalian macrophages. Arginase is an immune-regulatory enzyme, which can reduce the nitric oxide concentrations produced by activated macrophages and limit the availability of L-arginine, regulating the polyamines pathway, supporting the resistance of some pathogens to host defense mechanisms. Currently, we described by RNA-seq technology, the differential gene expression profile on polyamines pathway in promastigotes and axenic amastigotes regulated by arginase activity. Our goal in this project is to identify gene signature and differential gene expression profile between two mice strains, one highly susceptible and another less susceptible to L. amazonensis (La-WT) or L. amazonensis arginase knockout (La-arg-) infections. Further, validate and correlate the miRNAs participation that has been providing interesting data on understanding how regulation of gene expression is used by Leishmania to subvert the macrophage defenses.

News published in Agência FAPESP Newsletter about the scholarship:
A study investigating genetic mechanisms underlying resistance to leishmaniasis 
Articles published in other media outlets (20 total):
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UOL: Fatores genéticos que conferem resistência à leishmaniose são identificados (19/May/2020)
Mais Suas: Pesquisa aponta: mais de 7 milhões de elegíveis para receber a renda básica emergencial não têm acesso à internet (22/May/2020)
LabNetwork: Fatores genéticos que conferem resistência à leishmaniose são identificados (20/May/2020)
Blog Jornal da Mulher: Fatores genéticos que conferem resistência à leishmaniose são identificados (20/May/2020)
NewsLab online: Cientistas brasileiros descrevem fatores genéticos que conferem resistência à leishmaniose (19/May/2020)
JC Notícias (São Paulo, SP): Fatores genéticos que conferem resistência à leishmaniose são identificados (19/May/2020)
Portal da Enfermagem: Fatores genéticos que conferem resistência à leishmaniose são identificados (19/May/2020)
Central das Notícias: Fatores genéticos que conferem resistência à leishmaniose são identificados (19/May/2020)
NCYT Amazings (Espanha): Una investigación muestra los mecanismos genéticos que dotan de resistencia a la leishmaniasis (01/Apr/2020)
Health News: A study investigating genetic mechanisms underlying resistance to leishmaniasis (25/Mar/2020)
Bio1000 (China): Um estudo sobre o mecanismo genético subjacente da anti-leishmaniose (25/Mar/2020)
BioTech.WS: A study investigating genetic mechanisms underlying resistance to leishmaniasis (25/Mar/2020)
pidnews.org: A study investigating genetic mechanisms underlying resistance to leishmaniasis (25/Mar/2020)
Xinkangpm.com: Um estudo sobre o mecanismo genético subjacente da anti-leishmaniose (25/Mar/2020)
News Medical (Austrália): Study explores genetic mechanisms underlying resistance to leishmaniasis (24/Mar/2020)
Bioengineer (Reino Unido): A study investigating genetic mechanisms underlying resistance to leishmaniasis (24/Mar/2020)
Science Codex: A study investigating genetic mechanisms underlying resistance to leishmaniasis (24/Mar/2020)
Scienmag Science Magazine (Reino Unido): A study investigating genetic mechanisms underlying resistance to leishmaniasis (24/Mar/2020)
7thSpace: A study investigating genetic mechanisms underlying resistance to leishmaniasis (24/Mar/2020)
Diwou Biotech News (Austrália): A study investigating genetic mechanisms underlying resistance to leishmaniasis (24/Mar/2020)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AOKI, JULIANA IDE; LARANJEIRA-SILVA, MARIA FERNANDA; MUXEL, SANDRA MARCIA; FLOETER-WINTER, LUCILE MARIA. The impact of arginase activity on virulence factors of Leishmania amazonensis. Current Opinion in Microbiology, v. 52, p. 110-115, DEC 2019. Web of Science Citations: 0.
MARCIA MUXEL, SANDRA; MAMANI-HUANCA, MARICRUZ; AOKI, JULIANA IDE; ZAMPIERI, RICARDO ANDRADE; FLOETER-WINTER, LUCILE MARIA; LOPEZ-GONZALVEZ, ANGELES; BARBAS, CORAL. Metabolomic Profile of BALB/c Macrophages Infected with Leishmania amazonensis: Deciphering L-Arginine Metabolism. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 24 DEC 2019. Web of Science Citations: 0.
RIBEIRO FERNANDES, JULIANE CRISTINA; AOKI, JULIANA IDE; ACUNA, STEPHANIE MAIA; ZAMPIERI, RICARDO ANDRADE; MARKUS, REGINA P.; FLOETER-WINTER, LUCILE MARIA; MUXEL, SANDRA MARCIA. Melatonin and Leishmania amazonensis Infection Altered miR-294, miR-30e, and miR-302d Impacting on Tnf, Mcp-1, and Nos2 Expression. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 9, MAR 20 2019. Web of Science Citations: 2.
MUXEL, SANDRA MARCIA; ACUNA, STEPHANIE MAIA; AOKI, JULIANA IDE; ZAMPIERI, RICARDO ANDRADE; FLOETER-WINTER, LUCILE MARIA. Toll-Like Receptor and miRNA-let-7e Expression Alter the Inflammatory Response in Leishmania amazonensis-Infected Macrophages. FRONTIERS IN IMMUNOLOGY, v. 9, NOV 29 2018. Web of Science Citations: 3.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.