Extracellular matrix and oxidative stress role in lateral pterygoid muscle at orofacial dysfunction resulting of Pakinsonism experimental model

• Grant number: 17/23941-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): January 01, 2018
• Effective date (End): October 31, 2018
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Glauce Crivelaro Do Nascimento
• Grantee: Bruno Lima Malzone
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Musculoskeletal disorders, those caused by Parkinson's disease (PD), are highlighted by the high prevalence in the elderly population. It is known that this disorder stems from the direct and indirect effects on skeletal muscles and its main therapeutic drug (L-DOPA), but little is known about the consequences of this disease on masticatory muscles. This study aims to investigate the effect of Parkinson's disease, which will be induced by damage to dopaminergic neurons in the medial prosencephalic bundle (FPM), and administration of L-DOPA on the function and oxidative stress of lateral pterygoid muscle. Our hypothesis is that Parkinson's disease will induce changes in the expression of metalloproteinases and in the oxidative stress of the masticatory muscle to be studied. The influence of treatment with L-DOPA, a classic drug in the treatment of Parkinson's Disease, will also be evaluated. Male Wistar rats (200g) randomly divided into four groups: Control Group (CG n = 8), which will be animals not induced to Parkinson's Disease and will not receive chronic treatment with L-DOPA; Parkinson's Group (GP n = 8), which will be the animals induced to Parkinson's Disease by a lesion of dopaminergic neurons in the medial prosencephalic bundle (FPM - nigrostriatal pathway); Group L-DOPA (GL n = 8), which will be animals not induced to Parkinson's disease and who will receive chronic treatment with L-DOPA; and Parkinson + L-DOPA (GP + L n = 8), which will be the Parkinson's disease-induced animals and will receive chronic treatment with L-DOPA. Rats will be submitted on the 46th day after the start of the experiment to euthanasia, to obtain the left and right lateral pterygoid muscles for histochemical analysis of reactive oxygen species (EROS) and immunohistochemistry for type 2 metalloproteinase (MMP-2). (AU)