Evaluation of the low-frequency ultrasound potential for the sonodynamic therapy of skin cancer

• Grant number: 17/17442-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: January 01, 2018
• End date: August 31, 2019
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Renata Fonseca Vianna Lopez
• Grantee: Yugo Araújo Martins
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 14/22451-7 - Sustained drug delivery systems targeting the epithelial tissue, AP.TEM

Abstract

Sonodynamic therapy (SDT) is a therapeutic modality that has been studied for the non-invasive treatment of aggressive skin cancer such as melanoma. SDT involves the application of ultrasound on the tumor for the activation of sonosensitizing agent and the production of reactive oxygen species (ROS), which mediate the death of cancer cells. ROS generation is related to both the phenomenon of acoustic cavitation and the ultrasound frequency. Few studies have reported the effect of low-frequency ultrasound (LFU) on SDT, although LFU has extensively been studied in transdermal drug delivery. Therefore, LFU can be combined with SDT to facilitate skin penetration and activation of sonosensitizers. This work aims to investigate the influence of LFU on the SDT of melanoma associated or not with a sonosensitizer agent, chlorine-e6 (Ce6), topically administered, by utilizing both free and micelle form of Ce6. Cytotoxicity and in vitro production of ROS studies will be performed on murine melanoma cells under various conditions of LFU, Ce6 and micellar system application. In vitro skin penetration studies of Ce6 will be performed to investigate the influence of the micellar system and LFU on Ce6 penetration in skin. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS, YUGO A.; FONSECA, V, MARIA J.; PAVAN, THEO Z.; LOPEZ, V, RENATA F.. Bifunctional Therapeutic Application of Low-Frequency Ultrasound Associated with Zinc Phthalocyanine-Loaded Micelles. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 15, p. 8075-8095, 2020. (14/22451-7, 17/17442-7, 14/50928-2)

MARTINS, YUGO ARAUJO; PAVAN, THEO ZEFERINO; LOPEZ, RENATA FONSECA VIANNA. Sonodynamic therapy: Ultrasound parameters and in vitro experimental configurations. International Journal of Pharmaceutics, v. 610, DEC 15 2021. (17/17442-7, 14/22451-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARTINS, Yugo Araújo. Influence of the low-frequency ultrasound on the skin penetration of zinc phthalocyanine formulations and its potential for the sonodynamic therapy of skin tumors. 2019. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.