Therapeutic action of mesenchymal stem cells of the human bone marrow, marked with multimodal nanoparticles, in diabetic rats submitted to focal cerebral ischemia: study of cellular, molecular and functional mechanisms

• Grant number: 17/17868-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2018
• End date: February 28, 2019
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Lionel Fernel Gamarra Contreras
• Grantee: Yolanda Oliveira Pinto
• Host Institution: Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE). Sociedade Beneficente Israelita Brasileira Albert Einstein (SBIBAE). São Paulo , SP, Brazil

Abstract

The current project of scientific initiation aims to evaluate the marking of mesenchymal stem cells (MSC) of the human bone marrow with nanoparticles that have magnetic properties and double fluorescent labeling, which enable them being detected by magnetic resonance imaging and by in vitro (excitation / emission: 555 / 579nm) and in vivo (absorption / emission: 755 / 777nm) fluorescence imaging techniques looking forward the traceability, migration and viability of MSC after administration in animal models. Another strategy that will be used for the tracing is bioluminescence, since the MSC will be transfected with luciferase. Besides that, it will allow to observe the viability of the MSC. The present subproject make part of a regular aid project FAPESP N ° 16 / 21470-3, entitled "Therapeutic action of mesenchymal stem cells of the human bone marrow, marked with multimodal nanoparticles, in diabetic rats submitted to focal cerebral ischemia: study of cellular, molecular and functional mechanisms". The project will be divided into five stages: the first step consist in the study of multimodal nanoparticles (NPMM) stability in culture under the conditions that will be used in the labeling of MSC. The second step corresponds to the marking of the MSC with the NPMM. The third step will analyze the intracellular distribution by visualizing light microscopy in the presence of iron and fluorescence in vitro, using one of the fluorophores (555 / 579nm) for the detection of NPMM in MSC. In the fourth stage, the in vitro evaluation through Magnetic Resonance Imaging (MRI) will be performed. This phase will be realized by Dr. Javier Bustamante Mamani and accompanied by the student of scientific initiation. In the fifth stage, the evaluation by fluorescence tomography in the infrared and bioluminescence signal in the CTM will be realized. This phase will be accompanied by Dr. Helio Rodrigues da Silva, research associate of the project.

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VALLE, NICOLE M. E.; NUCCI, MARIANA P.; ALVES, ARIELLY H.; RODRIGUES, LUIZ D.; MAMANI, JAVIER B.; OLIVEIRA, FERNANDO A.; LOPES, CAIQUE S.; LOPES, ALEXANDRE T.; CARRENO, MARCELO N. P.; GAMARRA, LIONEL F.. Advances in Concentration Gradient Generation Approaches in a Microfluidic Device for Toxicity Analysis. CELLS, v. 11, n. 19, p. 56-pg., 2022-10-01. (16/21470-3, 17/17868-4, 19/21070-3)

ALVES, ARIELLY DA HORA; NUCCI, MARIANA PENTEADO; DO VALLE, NICOLE MASTANDREA ENNES; MISSINA, JULIANA MORAIS; MAMANI, JAVIER BUSTAMANTE; REGO, GABRIEL NERY ALBUQUERQUE; DIAS, OLIVIA FURIAMA METROPOLO; GARRIGOS, MURILO MONTENEGRO; DE OLIVEIRA, FERNANDO ANSELMO; GAMARRA, LIONEL FERNEL. Current overview of induced pluripotent stem cell-based blood-brain barrier-on-a-chip. WORLD JOURNAL OF STEM CELLS, v. 15, n. 6, p. 22-pg., 2023-06-26. (16/21470-3, 17/17868-4, 19/21070-3)

NUCCI, MARIANA P.; MAMANI, JAVIER B.; OLIVEIRA, FERNANDO A.; FILGUEIRAS, IGOR S.; ALVES, ARIELLY H.; THEINEL, MATHEUS H.; RODRIGUES, LUIZ D.; MARTI, LUCIANA; GAMARRA, LIONEL F.. Optimization of Multimodal Nanoparticles Internalization Process in Mesenchymal Stem Cells for Cell Therapy Studies. PHARMACEUTICS, v. 14, n. 6, p. 23-pg., 2022-06-01. (19/21070-3, 16/21470-3, 17/17868-4)

NUCCI, MARIANA P.; OLIVEIRA, FERNANDO A.; FERREIRA, JOAO M.; PINTO, YOLANDA O.; ALVES, ARIELLY H.; MAMANI, JAVIER B.; NUCCI, LEOPOLDO P.; VALLE, NICOLE M. E.; GAMARRA, LIONEL F.. ffect of Cell Therapy and Exercise Training in a Stroke Model, Considering the Cell Track by Molecular Image and Behavioral Analysi. CELLS, v. 11, n. 3, FEB 2022. (16/21470-3, 19/21070-3, 17/17868-4)

THEINEL, MATHEUS H.; NUCCI, MARIANA P.; ALVES, ARIELLY H.; DIAS, OLIVIA F. M.; MAMANI, JAVIER B.; GARRIGOS, MURILO M.; OLIVEIRA, FERNANDO A.; REGO, GABRIEL N. A.; VALLE, NICOLE M. E.; CIANCIARULLO, GABRIELA; et al. The Effects of Omega-3 Polyunsaturated Fatty Acids on Breast Cancer as a Preventive Measure or as an Adjunct to Conventional Treatments. NUTRIENTS, v. 15, n. 6, p. 34-pg., 2023-03-01. (16/21470-3, 17/17868-4, 19/21070-3)

GARRIGOS, MURILO MONTENEGRO; DE OLIVEIRA, FERNANDO ANSELMO; NUCCI, MARIANA PENTEADO; NUCCI, LEOPOLDO PENTEADO; ALVES, ARIELLY DA HORA; METROPOLO DIAS, OLIVIA FURIAMA; GAMARRA, LIONEL FERNEL. How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models. WORLD JOURNAL OF STEM CELLS, v. 14, n. 8, p. 22-pg., 2022-08-26. (16/21470-3, 17/17868-4, 19/21070-3)

GARRIGOS, MURILO M.; OLIVEIRA, FERNANDO A.; NUCCI, MARIANA P.; MAMANI, JAVIER B.; DIAS, OLIVIA F. M.; REGO, GABRIEL N. A.; JUNQUEIRA, MARA S.; COSTA, CICERO J. S.; SILVA, LUCAS R. R.; ALVES, ARIELLY H.; et al. Bioluminescence Imaging and ICP-MS Associated with SPION as a Tool for Hematopoietic Stem and Progenitor Cells Homing and Engraftment Evaluation. PHARMACEUTICS, v. 15, n. 3, p. 18-pg., 2023-03-01. (16/21470-3, 17/17868-4, 19/21070-3)

MURILO MONTENEGRO GARRIGÓS; FERNANDO ANSELMO DE OLIVEIRA; CÍCERO JÚLIO SILVA COSTA; LUCAS RENAN RODRIGUES; MARIANA PENTEADO NUCCI; ARIELLY DA HORA ALVES; JAVIER BUSTAMANTE MAMANI; GABRIEL NERY DE ALBUQUERQUE REGO; JUAN MATHEUS MUNOZ; LIONEL FERNEL GAMARRA. Assessing the toxicity of one-step-synthesized PEG-coated gold nanoparticles: in vitro and in vivo studies. Einstein (São Paulo), v. 22, 2024-05-20. (17/17868-4, 19/21070-3, 16/21470-3)