In vitro metabolism of steroidal alkaloids from Solanum paniculatum l. (Solanaceae)

• Grant number: 17/19330-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2018
• End date: August 31, 2019
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Norberto Peporine Lopes
• Grantee: Matheus Coutinho Gazolla
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 14/50265-3 - Distribution and metabolism of natural and synthetic xenobiotics: from the comprehension of reactional process to tissue imaging generation, AP.BTA.TEM

Abstract

The species Solanum paniculatum L. belongs to the family Solanaceae and popularly called jurubeba. Used in traditional medicine for the treatment of gastric and hepatic dysfunctions, it is also present in herbal medicines for this purpose. The production of steroidal alkaloids such as jurubidina and jurubina is characteristic of plants of the genus Solanum, which are attributed to the aforementioned biological activities due to the presence of the amine function in the molecule. In addition, these substances demonstrate membrane properties in function of the steroidal skeleton. Several in vitro methodologies such as the use of hepatic microsomes and biomimetic oxidation assays with metalloporphyrins were developed with the aim of mimicking the in vivo phase I and II reactions promoted in the liver in the metabolism process. When associated with sensitive techniques such as mass spectrometry and nuclear magnetic resonance provide relevant information on the possible metabolites formed that combined with other techniques are also able to elucidate the reactive kinetic aspects of metabolism. Thus, current techniques for studying in vitro metabolism in conjunction with spectroscopic analyzes appear as a tool for analysis and collaboration for a complete study of administration, absorption, distribution and elimination of natural products, especially of Solanaceae family species. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GAZOLLA, MATHEUS COUTINHO; MAURIZ MARQUES, LUCAS MACIEL; GAMA E SILVA, MARIANA; MOURA FONTES ARAUJO, MARIA TAIS; MENDES, ROSEMAIRY LUCIANE; GUEDES DA SILVA ALMEIDA, JACKSON ROBERTO; VESSECCHI, RICARDO; LOPES, NORBERTO PEPORINE. Characterization of 3-aminospirostane alkaloids from roots of Solanum paniculatum L. with hepatoprotective activity. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v. 34, p. 9-pg., 2020-02-11. (17/19330-1, 14/23604-1, 14/50265-3)

GAZOLLA, MATHEUS COUTINHO; MAURIZ MARQUES, LUCAS MACIEL; GAMA E SILVA, MARIANA; MOURA FONTES ARAUJO, MARIA TAIS; MENDES, ROSEMAIRY LUCIANE; GUEDES DA SILVA ALMEIDA, JACKSON ROBERTO; VESSECCHI, RICARDO; LOPES, NORBERTO PEPORINE. Characterization of 3-aminospirostane alkaloids from roots of Solanum paniculatum L. with hepatoprotective activity. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, FEB 2020. (14/50265-3, 17/19330-1, 14/23604-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GAZOLLA, Matheus Coutinho. Structural characterization, hepatoprotective activity and in vitro metabolism of 3-aminospirostanes alkaloids from roots of Solanum paniculatum L. (Solanaceae). 2020. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.