Investigation of the potential of filamentous fungi to biotransform the ent-polyalthic acid

• Grant number: 17/20397-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2018
• End date: March 31, 2021
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Niege Araçari Jacometti Cardoso Furtado
• Grantee: Lívia Maria Fernandes
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

This project aims to investigate different cultivation conditions of five filamentous fungi in order to evaluate the influence of the culture medium and the incubation time on the biotransformation reactions of ent-polyalthic acid. It is also intended to select one or two filamentous fungi to biotransform this diterpene. The ent-polyalthic acid is a furan diterpene of labdane skeleton already identified as a constituent of the oleoresins of different Copaifera sp. species, being the major constituent of the Copaifera duckei oleoresin. Oleoresins from Copaifera species are marketed all over Brazil, being considered the raw material of vegetable origin most used in folk medicine in Brazil and are also exported to other countries. Filamentous fungi have been extremely useful in biotransformation processes contributing to the synthesis and obtaining of bioactive molecule derivatives, as well as to metabolism studies constituting an important tool to obtain the metabolites of interest in enough quantities to carry out toxicological and biological assays. In order to reach the proposed objectives, five filamentous fungi will be cultivated under different conditions, extracts will be obtained from the culture broths and mycelia and all the extracts will be submitted to the chromatographic analysis using thin-layer chromatography and also high-performance liquid chromatography coupled to DAD and Corona CAD detectors. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUSA TEIXEIRA, M., V; FERNANDES, L. M.; DE PAULA, V. STEFANELLI; FERREIRA, A. G.; JACOMETTI CARDOSO FURTADO, N. A.. Ent-hardwickiic acid from C. pubiflora and its microbial metabolites are more potent than fluconazole in vitro against Candida glabrata. Letters in Applied Microbiology, v. 74, n. 4, p. 8-pg., 2022-01-17. (11/13630-7, 16/25201-7, 17/20397-3, 17/20071-0)