Taxonomic and functional evaluations of microbial community involved in methane cycling of Brazilian Amazon soils

• Grant number: 17/26138-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2018
• End date: September 30, 2019
• Field of knowledge: Biological Sciences - Microbiology
• Agreement: NSF - Dimensions of Biodiversity and BIOTA
• Principal Investigator: Tsai Siu Mui
• Grantee: Fabiana da Silva Paula
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 14/50320-4 - Dimensions US-BIOTA - São Paulo: collaborative research: integrating dimensions of microbial biodiversity across land use change in tropical forests, AP.BTA.TEM

Abstract

This proposal aims at studying of Bacteria/Archaea involved in consumption/emission of methane in soilsunder different land uses in comparison with natural forest. Here, we will investigate several metabolic features through in silico modeling and functional integration of -omics data. The assessment of functional fractions of communities will be mediated by SIP analysis and also by isolation of organisms involved in methane cycling. Based on both resources, SIP-labeled DNA and isolates will serve to metabolic reconstruction, generating an innovative dataset of present and active genes on soils under different use. The integration of such data with other dimensions of biodiversity in Amazonia might lead to a model, whose predictive potential will be validated against a set of experimentally determined growth rates and growth phenotypic data. Furthermore, evidence synthesis from proteomics, phenomics, physiology and metabolic modeling data will allow us to reveal possible drawbacks of methane-dependent changes in gene expression on the overall metabolic network of the methane cycling. This study will bring a valuable platform for further investigations on the bacterial cellular functional states for the methane consumption/emission microbial processes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GONTIJO, JULIA B.; PAULA, FABIANA S.; VENTURINI, ANDRESSA M.; YOSHIURA, CAIO A.; BORGES, CLOVIS D.; MOURA, JOSE MAURO S.; BOHANNAN, BRENDAN J. M.; NUSSLEIN, KLAUS; MAZZA RODRIGUES, JORGE L.; TSAI, SIU M.. Not just a methane source: Amazonian floodplain sediments harbour a high diversity of methanotrophs with different metabolic capabilities. Molecular Ecology, v. 30, n. 11, p. 2560-2572, JUN 2021. (14/50320-4, 17/26138-0, 18/14974-0, 17/09643-2, 15/13546-7, 19/25931-3)

GONTIJO, JULIA B.; PAULA, FABIANA S.; VENTURINI, ANDRESSA M.; MANDRO, JESSICA A.; BODELIER, PAUL L. E.; TSAI, SIU M.. Insights into the Genomic Potential of a Methylocystis sp. from Amazonian Floodplain Sediments. MICROORGANISMS, v. 10, n. 9, p. 11-pg., 2022-09-01. (18/14974-0, 14/50320-4, 17/26138-0, 19/25924-7, 19/25931-3)

VENTURINI, ANDRESSA M.; DIAS, NAISSA M. S.; GONTIJO, JULIA B.; YOSHIURA, CAIO A.; PAULA, FABIANA S.; MEYER, KYLE M.; NAKAMURA, FERNANDA M.; DA FRANCA, ALINE G.; BORGES, CLOVIS D.; BARLOW, JOS; et al. Increased soil moisture intensifies the impacts of forest-to-pasture conversion on methane emissions and methane-cycling communities in the Eastern Amazon. Environmental Research, v. 212, p. 13-pg., 2022-04-06. (14/50320-4, 15/13546-7, 15/08564-6, 19/25931-3, 17/09616-5, 17/26138-0, 16/11268-2, 17/09643-2, 17/09952-5, 15/12282-6, 18/14974-0)