Taxonomic and functional depiction of the microbial community involved in methane transformations in soils of the Brazilian Amazonia

• Grant number: 19/25931-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): February 01, 2020
• Effective date (End): March 31, 2021
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Acordo de Cooperação: NSF - Dimensions of Biodiversity and BIOTA
• Principal Investigator: Tsai Siu Mui
• Grantee: Andressa Monteiro Venturini
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 14/50320-4 - Dimensions US-BIOTA - São Paulo: collaborative research: integrating dimensions of microbial biodiversity across land use change in tropical forests, AP.BTA.TEM

Abstract

This proposal, integrated to the collaborative project "Dimensions US-BIOTA-São Paulo: Integrating dimensions of microbial biodiversity across land use change in tropical forests", aims at studying Bacteria/Archaea involved in the consumption and emission of methane in soils under different land-uses in the Brazilian Amazon in comparison with natural forest. Here we will investigate several metabolic features through in silico modeling and functional integration of omics data. The assessment of the functional fractions of the communities will be mediated by stable-isotope probing (SIP) analysis and also by isolation of organisms involved in methane cycling. Based on both resources, SIP-labeled DNA and isolates will serve for metabolic reconstruction, generating an innovative dataset on active genes present in soils under different uses. The integration of such data with other dimensions of biodiversity in Amazonia might lead to a model, whose predictive potential will be validated against a set of experimentally determined growth rates and phenotypic data. Furthermore, evidence synthesis from proteomics, phenomics, physiology, and metabolic modeling data will allow us to reveal possible drawbacks of methane-dependent changes in gene expression on the overall metabolic network of methane cycling. This study will bring a valuable platform for further investigations on the microbial functional states for the methane consumption and emission processes. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

YOSHIURA, CAIO AUGUSTO; VENTURINI, ANDRESSA MONTEIRO; PEREZ BRAGA, LUCAS PALMA; DA FRANCA, ALINE GIOVANA; CATANHO PEREIRA DE LYRA, MARIA DO CARMO; TSAI, SIU MUI; RODRIGUES, JORGE LUIZ MAZZA. Responses of Low-Cost Input Combinations on the Microbial Structure of the Maize Rhizosphere for Greenhouse Gas Mitigation and Plant Biomass Production. FRONTIERS IN PLANT SCIENCE, v. 12, JUN 30 2021. (13/22845-2, 15/13546-7, 15/08564-6, 16/11268-2, 14/50320-4, 19/25931-3)

VENTURINI, ANDRESSA M.; DIAS, NAISSA M. S.; GONTIJO, JULIA B.; YOSHIURA, CAIO A.; PAULA, FABIANA S.; MEYER, KYLE M.; NAKAMURA, FERNANDA M.; DA FRANCA, ALINE G.; BORGES, CLOVIS D.; BARLOW, JOS; et al. Increased soil moisture intensifies the impacts of forest-to-pasture conversion on methane emissions and methane-cycling communities in the Eastern Amazon. Environmental Research, v. 212, p. 13-pg., 2022-04-06. (14/50320-4, 15/13546-7, 15/08564-6, 19/25931-3, 17/09616-5, 17/26138-0, 16/11268-2, 17/09643-2, 17/09952-5, 15/12282-6, 18/14974-0)

BIELUCZYK, WANDERLEI; ASSELTA, FERNANDA OMETTO; NAVROSKI, DEISI; GONTIJO, JULIA BRANDAO; VENTURINI, ANDRESSA MONTEIRO; MENDES, LUCAS WILLIAM; SIMON, CARLA PENHA; DE CAMARGO, PLINIO BARBOSA; TADINI, AMANDA MARIA; -NETO, LADISLAU MARTIN; et al. Linking above and belowground carbon sequestration, soil organic matter properties, and soil health in Brazilian Atlantic Forest restoration. Journal of Environmental Management, v. 344, p. 15-pg., 2023-07-15. (19/25931-3, 20/15230-5, 18/14974-0, 18/19000-4, 19/16822-6)

GONTIJO, JULIA B.; PAULA, FABIANA S.; VENTURINI, ANDRESSA M.; YOSHIURA, CAIO A.; BORGES, CLOVIS D.; MOURA, JOSE MAURO S.; BOHANNAN, BRENDAN J. M.; NUSSLEIN, KLAUS; MAZZA RODRIGUES, JORGE L.; TSAI, SIU M.. Not just a methane source: Amazonian floodplain sediments harbour a high diversity of methanotrophs with different metabolic capabilities. Molecular Ecology, v. 30, n. 11, p. 2560-2572, JUN 2021. (14/50320-4, 17/26138-0, 18/14974-0, 17/09643-2, 15/13546-7, 19/25931-3)

MANDRO, JESSICA A.; NAKAMURA, FERNANDA M.; GONTIJO, JULIA B.; TSAI, SIU M.; VENTURINI, ANDRESSA M.. Metagenome-Assembled Genomes from Amazonian Soil Microbial Consortia. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 11, n. 11, p. 5-pg., 2022-10-27. (14/50320-4, 15/12282-6, 15/23758-1, 15/13546-7, 19/26029-1, 18/14974-0, 19/25931-3)

VENTURINI, ANDRESSA M.; GONTIJO, JULIA B.; DA FRANCA, ALINE G.; MOURA, JOSE M. S.; NUSSLEIN, KLAUS; BOHANNAN, BRENDAN J. M.; RODRIGUES, JORGE L. M.; TSAI, SIU M.. Metagenomes from Eastern Brazilian Amazonian Floodplains in the Wet and Dry Seasons. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 11, n. 8, p. 4-pg., 2022-07-19. (18/14974-0, 14/50320-4, 19/25924-7, 19/25931-3)

GONTIJO, JULIA B.; PAULA, FABIANA S.; VENTURINI, ANDRESSA M.; MANDRO, JESSICA A.; BODELIER, PAUL L. E.; TSAI, SIU M.. Insights into the Genomic Potential of a Methylocystis sp. from Amazonian Floodplain Sediments. MICROORGANISMS, v. 10, n. 9, p. 11-pg., 2022-09-01. (18/14974-0, 14/50320-4, 17/26138-0, 19/25924-7, 19/25931-3)