Study of lipid rafts through the interaction between membrane models and chitosan and cholesterol oxidase

• Grant number: 18/00878-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2018
• End date: June 30, 2020
• Field of knowledge: Physical Sciences and Mathematics - Physics
• Principal Investigator: Osvaldo Novais de Oliveira Junior
• Grantee: Andressa Ribeiro Pereira
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/14262-7 - Nanostructured films from biologically-relevant materials, AP.TEM

Abstract

The formation of lipid rafts was initially proposed based on the preferential interactions between sphingolipids and cholesterol. While the basic Biophysics for the composition of lipid rafts is explained by simple principles, the theory for the selective incorporation of proteins into more ordered domains remains a mystery. Lipid rafts are usually located in regions of high concentration of proteins and lipids and have been associated with important biological processes. It is known that different lipid domains have different physicochemical properties, defined according to their packing and depending on the interactions between saturated lipids and cholesterol. Despite the importance of lipid rafts, little is known about the actual mechanism of its effect on cellular function. Thus, in this project we intend to study the lipid rafts and their interactions with biomolecules such as the enzyme cholesterol oxidase and the biopolymer chitosan. With the enzyme we shall study the influence of cholesterol on the membranes, since cholesterol oxidase is responsible for the oxidation of cholesterol. From the studies with chitosan, we expect to determine the permeation mechanism of the membrane, which is relevant for the use of chitosan as drug carrier. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEDRO, RAFAEL DE OLIVEIRA; PEREIRA, ANDRESSA RIBEIRO; OLIVEIRA, OSVALDO N.; MIRANDA, PAULO BARBEITAS. Interaction of chitosan derivatives with cell membrane models in a biologically relevant medium. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 192, AUG 2020. (18/00878-0, 18/02819-0, 17/03503-4)

PEREIRA, ANDRESSA R.; FIAMINGO, ANDERSON; PEDRO, RAFAEL DE O.; CAMPANA-FILHO, SERGIO P.; MIRANDA, PAULO B.; OLIVEIRA JR, OSVALDO N.. Enhanced chitosan effects on cell membrane models made with lipid raft monolayers. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 193, SEP 2020. (17/03503-4, 13/14262-7, 18/00878-0, 16/09720-4)

PEREIRA, ANDRESSA R.; SHIMIZU, FLAVIO M.; OLIVEIRA JR, OSVALDO N.. Cholesterol modulates the interaction between paclitaxel and Langmuir monolayers simulating cell membranes. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 205, SEP 2021. (18/00878-0, 18/22214-6)

MODENEZ, IAGO A.; MACEDO, LUCYANO J. A.; MELO, ANTONIO F. A. A.; PEREIRA, ANDRESSA R.; OLIVEIRA JR, OSVALDO N.; CRESPILHO, FRANK N.. Nanosized non-proteinaceous complexes III and IV mimicking electron transfer of mitochondrial respiratory chain. Journal of Colloid and Interface Science, v. 599, p. 198-206, OCT 2021. (19/12053-8, 18/22214-6, 17/03879-4, 17/20493-2, 19/15333-1, 18/00878-0)

JOCHELAVICIUS, KAREN; PEREIRA, ANDRESSA R.; FIAMINGO, ANDERSON; NOBRE, THATYANE M.; CAMPANA-FILHO, SERGIO P.; OLIVEIRA JR, OSVALDO N.. Chitosan effects on monolayers of zwitterionic, anionic and a natural lipid extract from E. coli at physiological pH. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 209, n. 2, JAN 2022. (18/22214-6, 18/00878-0)