CHARACTERIZATION OF THE INFLAMMATORY RESPONSE PROFILE IN THE PERIVASCULAR ADIPOSE TISSUE ASSOCIATED WITH CORONARY ARTERY DISEASE: AN AUTOPSY STUDY

• Grant number: 17/24066-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2018
• End date: July 31, 2022
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Claudia Kimie Suemoto
• Grantee: Daniela Souza Farias Itao
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Introduction: Coronary artery disease (CAD) is the main cause of morbimortality in the world. In a previous study, we found a positive correlation between the number of macrophages in the perivascular adipose tissue (PAT) and the percentage of obstruction of the atherosclerotic plaque, and an association between B-lymphocytes and unstable lesions. However, it is necessary to elucidate the inflammatory response profile in the PAT associated with CAD. The analysis of the macrophage polarization was performed in few studies with surgical samples, which could initiate an inflammatory process. Moreover, the main lineages of the T and B-lymphocytes in human PAT have not been research yet. Aim: To investigate the association between the inflammation response profile in periplaque PAT adjacent to the atherosclerosis plaque in coronary arteries, and to compare this inflammatory response profile with PAT adjacent to the coronary artery without atherosclerosis (distal PAT), using autopsy material. Methods: This cross-sectional study will be conducted in 82 samples of participants previously collected in São Paulo Autopsy Service of the University of São Paulo. The coronary arteries were dissected, fixed in 4% paraformaldehyde, and microscopically evaluated using morphometric measures. The atherosclerotic plaques were classified in terms of instability according to the American Heart Association criteria. The samples of periplaque and distal PAT will be submitted to immunohistochemistry technique to identify macrophage polarization M1 (CD11c+) e M2 (CD206+), and double-marking to identify Th1 lymphocytes (CD4+CD183+), Tregs (CD4+FoxP3+), B1 (CD20+CD43+) and B2 (CD20+CD43-). The slides will be scanned and the inflammatory cells will be counted in 20 random fields systematically distributed. The inflammatory cells will be compared using linear regression models adjusted to possible confusing factors.

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BERRY, KACEY; FARIAS-ITAO, DANIELA S.; GRINBERG, LEA T.; PLOWEY, EDWARD D.; SCHNEIDER, JULIE A.; RODRIGUEZ, ROBERTA D.; SUEMOTO, CLAUDIA K.; BUCKWALTER, MARION S.. and T Lymphocyte Densities Remain Stable With Age in Human Corte. ASN NEURO, v. 13, MAY 2021. (17/24066-1)

YAHAGI-ESTEVAM, MARISTELLA; FARIAS-ITAO, DANIELA SOUZA; PARAIZO LEIT, RENATA ELAINE; RODRIGUEZ, ROBERTA DIEHL; PASQUALUCCI, CARLOS AUGUSTO; NITRINI, RICARDO; JACOB-FILHO, WILSON; POWER, MELINDA C.; SUEMOTO, CLAUDIA KIMIE. The Potential Role of Selection Bias in the Association Between Coronary Atherosclerosis and Cognitive Impairment. JOURNAL OF ALZHEIMER'S DISEASE, v. 93, n. 4, p. 10-pg., 2023-01-01. (17/11313-0, 13/12290-3, 17/24066-1, 13/00335-2)

PARADELA, REGINA SILVA; FARIAS-ITAO, DANIELA SOUZA; LEITE, RENATA E. P.; PASQUALUCCI, CARLOS A.; GRINBERG, LEA T.; NASLAVSKY, MICHEL SATYA; ZATZ, MAYANA; NITRINI, RICARDO; JACOB-FILHO, WILSON; SUEMOTO, CLAUDIA KIMIE. Apolipoprotein E 62 allele is associated with lower risk of carotid artery obstruction in a population-based autopsy study. JOURNAL OF STROKE & CEREBROVASCULAR DISEASES, v. 32, n. 9, p. 6-pg., 2023-09-01. (09/09134-4, 16/24326-0, 17/24066-1, 06/55318-1)

SOUZA, JONAS GORDILHO; FARIAS-ITAO, DANIELA SOUZA; ALIBERTI, MARLON J. R.; BERTOLA, LAISS; ANDRADE, FABIOLA BOF DE; LIMA-COSTA, MARIA FERNANDA; FERRI, CLEUSA P.; SUEMOTO, CLAUDIA K.. Social Isolation, Loneliness, and Cognitive Performance in Older Adults: Evidence From the ELSI-Brazil Study. AMERICAN JOURNAL OF GERIATRIC PSYCHIATRY, v. 31, n. 8, p. 11-pg., 2023-06-29. (17/24066-1)

J.G. SOUZA; D.S. FARIAS-ITAO; M.J.R. ALIBERTI; T.S. ALEXANDRE; C. SZLEJF; C.P. FERRI; M.F. LIMA-COSTA; C.K. SUEMOTO. Diabetes, hemoglobin A1c, and cognitive performance in older adults: is there any impact of frailty? Evidence from the ELSI-Brazil study. Brazilian Journal of Medical and Biological Research, v. 57, 2024-02-19. (17/24066-1)

FARIAS-ITAO, DANIELA SOUZA; PASQUALUCCI, CARLOS AUGUSTO; DE ANDRADE, RENATO ARAUJO; FERRAZ DA SILVA, LUIZ FERNANDO; YAHAGI-ESTEVAM, MARISTELLA; GELAS LAGE, SILVIA HELENA; PARAIZO LEITE, RENATA ELAINE; CAMPO, ALEXANDRE BRINCALEPE; SUEMOTO, CLAUDIA KIMIE. Macrophage Polarization in the Perivascular Fat Was Associated With Coronary Atherosclerosis. JOURNAL OF THE AMERICAN HEART ASSOCIATION, v. 11, n. 6, p. 30-pg., 2022-03-15. (13/12290-3, 17/11313-0, 13/00335-2, 17/24066-1)

CICILIATI, ALINE MARIA M.; ADRIAZOLA, IZABELA ONO; SOUZA FARIAS-ITAO, DANIELA; PASQUALUCCI, CARLOS AUGUSTO; LEITE, RENATA ELAINE PARAIZO; NITRINI, RICARDO; GRINBERG, LEA T.; JACOB-FILHO, WILSON; SUEMOTO, CLAUDIA KIMIE. Severe Dementia Predicts Weight Loss by the Time of Death. FRONTIERS IN NEUROLOGY, v. 12, MAY 14 2021. (13/12290-3, 06/55318-1, 17/24066-1)

DOS SANTOS, ADRIANA NANCY MEDEIROS; FARIAS-IAO, DANIELA SOUZA; BENSENOR, ISABELA M.; GOULART, ALESSANDRA C.; BRUNONI, ANDRE R.; VIANA, MARIA CARMEN; LOTUFO, PAULO A.; SUEMOTO, CLAUDIA KIMIE. Potentially inappropriate medications and cognitive performance: cross-sectional results from the ELSA-Brasil study. EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY, v. 79, n. 7, p. 8-pg., 2023-05-10. (17/24066-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ITAO, Daniela Souza Farias. Characterization of the inflammatory response profile in the perivascular adipose tissue associated with coronary artery disease: an autopsy study. 2022. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.