Abundance of communities methanotrophic bacteria in corn (Zea mays) and cowpea (Vigna unguiculata) rizosphere cultivated on Amazon dark earth

• Grant number: 18/08193-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2018
• End date: December 31, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Tsai Siu Mui
• Grantee: Lucas Roberto de Oliveira
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 14/50320-4 - Dimensions US-BIOTA - São Paulo: collaborative research: integrating dimensions of microbial biodiversity across land use change in tropical forests, AP.BTA.TEM

Abstract

Among the many functions performed, the Amazon forest acts as a regulator of the atmospheric gases flow, especially those related to the greenhouse effect, such as methane gas, which is the second most important related to this phenomenon, and which has a very high retention power, higher than carbon dioxide. In the biogeochemical cycle of this gas have important participation of the community of microbial, which are regulated by arches that produce methane and bacteria that consume it. The objective of this work is to determine the effect of rhizosphere on the abundance of bacteria and on its methanotrophic activity when cultivating maize and cowpea in Amazon Dark Earth, an Amazonian anthroposol known for its high fertility and microbial diversity. In this context, the real-time quantitative PCR technique will be used to quantify the bacterial communities temporally by 16S rRNA gene analysis and the methanotrophic genes by the pmoA and mmoX genes.