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DNA damage in women with dyslipidemia before and after the nutritional intervention

Grant number: 18/00501-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2018
Effective date (End): December 31, 2018
Field of knowledge:Health Sciences - Nutrition
Principal Investigator:Raquel Alves dos Santos
Grantee:Pâmela Cristina Oliveira Januário
Home Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

Dyslipidemias are metabolic alterations characterized by change in blood concentration and accumulation of one or more class of lipoproteins. This metabolic disorder is closely linked to the risk of cardiovascular disease. Although many dyslipidemias are caused by genetic factors, the lifestyle allied to an unbalanced diet and sedentary lifestyle are still the major causes of this metabolic derangement. In a situation of dyslipidemia, the excess of free fatty acids in the blood ends up being an easy target for the reactive oxygen species, leading to a lipoperoxidation, which in turn generates by-products able to cause damage to other macromolecules, including the DNA. The relationship between some metabolic diseases such as diabetes, for example, and increased DNA damage is already known, but when it comes specifically to dyslipidemias alone, there is little information in the literature from studies in human populations. The aim of this research project is to evaluate, using the cytome micronucleus assay form buccal cells, the frequency of micronuclei in dyslipidemic women treated at the Nutrition Clinic of the University of Franca. The study will include 100 dyslipidemic women (hypertriglyceridemia and / or hypercholesterolemia) who will be submitted for a period of three months to a nutritional intervention to control dyslipidemia. The frequency of micronuclei (MN) in oral mucosal cells as well as blood levels of total cholesterol, HDL (high density lipoprotein), VLDL (very low density lipoprotein), LDL (low density lipoprotein) lipoprotein), triglycerides, aspartate aminotransferase (ASP) and alanine aminotransferase (ALT) will be analyzed before and after nutritional intervention. The correlation between the MN frequency and the biochemical variables evaluated will also be analyzed.