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Whole-genome DNA methylation profiling before and after different obesity treatments

Grant number: 16/05638-1
Support type:Regular Research Grants
Duration: September 01, 2016 - August 31, 2018
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal Investigator:Carla Barbosa Nonino
Grantee:Carla Barbosa Nonino
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Assoc. researchers:Julio Sérgio Marchini


Regardless of the strategy used in the control/treatment of obesity, the individual response to interventions is highly variable, and the genetic variation between individuals explains the variety of physiological responses in the same environment, explaining why some individuals are more likely to win/lose weight than others in the same environmental conditions. The aim of this study is to identify the changes in DNA methylation pattern in blood and adipose tissue before and after different treatment for weight loss: bariatric surgery and dietary intervention. This study will enroll 30 women from a mixed population, aged 18 to 60 years which will be randomized into two groups: Group Bariatric Surgery (G1): composed of 10 patients with severe obesity (BMI>40kg/m²); Group Dietary Intervention (G2): composed of 10 patients with severe obesity (BMI>40kg/m²) subjected to a hypocaloric dietary intervention program (1200 kcal/day) and Control group (G3), consisting of 10 normal weigh women (BMI between 18.5 and 24.9 kg/m²). The patients will be submitted to nutritional evaluation, analysis of body composition, food intake analysis, peripheral blood collection for genetic (DNA methylation) and biochemical analysis, and subcutaneous abdominal adipose collection for genetic analysis. After six months all procedures will be performed again in G1, and after six weeks for G2. t test, linear regression models and Pearson correlation will be used in statistical analyses. The analysis of whole methylome will be performed by bioinformatics tools including specific software.It will be admitted level of significance at p<0.05. (AU)

Articles published in Agência FAPESP Newsletter about the research grant
Drivers of the obesity epidemic in Brazil 

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NICOLETTI, C. F.; CORTES-OLIVEIRA, C.; NORONHA, N. Y.; PINHEL, M. A. S.; DANTAS, W. S.; JACOME, A.; MARCHINI, J. S.; GUALANO, B.; CRUJEIRAS, A. B.; NONINO, C. B. DNA methylation pattern changes following a short-term hypocaloric diet in women with obesity. European Journal of Clinical Nutrition, MAY 2020. Web of Science Citations: 0.
NICOLETTI, C. F. .; PINHEL, M. A. S.; NORONHA, N. Y.; DE OLIVEIRA, B. A.; SALGADO JUNIOR, W.; JACOME, A.; DIAZ-LAGARES, A.; CASANUEVA, F.; CRUJEIRAS, A. B.; NONINO, C. B. Altered pathways in methylome and transcriptome longitudinal analysis of normal weight and bariatric surgery women. SCIENTIFIC REPORTS, v. 10, n. 1 APR 15 2020. Web of Science Citations: 0.
NICOLETTI, CAROLINA F.; PINHEL, MARCELA S.; NORONHA, NATALIA Y.; JACOME, AMALIA; CRUJEIRAS, ANA B.; BARBOSA NONINO, CARLA. Association of MFSD3 promoter methylation level and weight regain after gastric bypass: Assessment for 3 y after surgery. NUTRITION, v. 70, FEB 2020. Web of Science Citations: 0.
NICOLETTI, C. F.; PINHEL, M. A. S.; DIAZ-LAGARES, A.; CASANUEVA, F. F.; JACOME, A.; PINHANELLI, V. C.; DE OLIVEIRA, B. A. P.; CRUJEIRAS, A. B.; NONINO, C. B. DNA methylation screening after roux-en Y gastric bypass reveals the epigenetic signature stems from genes related to the surgery per se. BMC MEDICAL GENOMICS, v. 12, MAY 27 2019. Web of Science Citations: 0.
NICOLETTI, CAROLINA F.; CORTES-OLIVEIRA, CRISTIANA; PINHEL, MARCELA A. S.; NONINO, CARLA B. Bariatric Surgery and Precision Nutrition. NUTRIENTS, v. 9, n. 9 SEP 2017. Web of Science Citations: 10.

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