Role of electrical activaton of the carotid sinus nerve, in conscious rats, in the attenuation of local and systemic inflammatory response elicited by periodontitis.

• Grant number: 18/10455-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): July 01, 2018
• Effective date (End): October 31, 2020
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Helio Cesar Salgado
• Grantee: Aline Barbosa Ribeiro
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 13/20549-7 - New insights in cardiovascular regulation under physiological and pathophysiological condition, AP.TEM
• Associated scholarship(s): 18/23703-0 - Mechanisms of periodontal inflammation and cardiovascular outcomes, BE.EP.PD

Abstract

Our laboratory has shown that the baroreflex was able to modulate local inflammatory responses, for example, in the joint, through sympathetic inhibition (Bassi et al., 2015), while the carotid chemoreflex was able to modulate the systemic inflammatory responses involving the autonomic sympathetic and parasympathetic nervous system (Santos - Almeida et al., 2017). On the other hand, periodontitis, a disease that affects 30-60% of the population, exhibits local (jaw) and systemic inflammatory characteristics (vascular dysfunction / arterial hypertension and inflammatory bowel diseases); at the present moment periodontitis does not present an effective therapy, except surgical approaches. Thus, motivated by clinical studies of carotid baroreflex activation for the treatment of resistant hypertension (Grassi et al., 2016), as well as unilateral carotid body resection (Narkiewicz et al., 2016), it is proposed in the present study to examine the role of electrical activation of the carotid sinus nerve, in conscious rat, with the purpose of characterizing whether the baro- and the chemoreflex are capable of modulating the local and/or systemic inflammatory responses, observed after ligature-induced periodontitis model of the jaw. Wistar Hannover rats will be submitted, under anaesthesia, to mandibular ligature for induction of periodontitis and implantation of a pair of electrodes around the carotid sinus nerve. Thus, each animal will have the carotid nerve chronically (09 days) stimulated every day at 17:00, with the following parameters: 0.5 mA; 30; 0.25 ms duration, for 15 minutes. On the eighth day after ligation of the jaw and implantation of the electrodes, the animals will have implanted, under anesthesia, a catheter into the femoral artery, in order to record blood pressure and heart rate on the following day; these records have the purpose to verify the efficacy of the electrical stimulation of the carotid nerve, by means of the hypotensive and bradycardic responses. Also, blood samples (1 mL) will be collected for analysis of plasma cytokines. Next, the animals will be anaesthetized for bone and gingival biopsies, and then submitted to decapitation, byguillotine, to collect the intestinal epithelium, which will be frozen for further analysis of tissue cytokines.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BROGNARA, FERNANDA; CASTANIA, JACI AIRTON; RIBEIRO, ALINE BARBOSA; SANTOS-JUNIOR, NILTON NASCIMENTO; SALGADO, HELIO CESAR. The Bezold-Jarisch Reflex and The Inflammatory Response Modulation in Unanesthetized Endotoxemic Rats. FRONTIERS IN PHYSIOLOGY, v. 12, SEP 20 2021. (13/20549-7, 20/06043-7, 18/10455-9, 18/20939-3, 17/05163-6)

RIBEIRO, ALINE BARBOSA; BROGNARA, FERNANDA; DA SILVA, JOSIANE FERNANDES; CASTANIA, JACI AIRTON; FERNANDES, PATRICIA GARANI; TOSTES, RITA C.; SALGADO, HELIO CESAR. Carotid sinus nerve stimulation attenuates alveolar bone loss and inflammation in experimental periodontitis. SCIENTIFIC REPORTS, v. 10, n. 1, NOV 6 2020. (18/10455-9, 17/05163-6)

RIBEIRO, ALINE BARBOSA; SANTOS-JUNIOR, NILTON NASCIMENTO; LUIZ, JOAO PAULO MESQUITA; DE OLIVEIRA, MAURO; KANASHIRO, ALEXANDRE; TAIRA, THAISE MAYUMI; FUKADA, SANDRA YASUYO; ALVES-FILHO, JOSE CARLOS; FAZAN JUNIOR, RUBENS; SALGADO, HELIO CESAR. Cardiovascular and Autonomic Dysfunction in Murine Ligature-Induced Periodontitis. SCIENTIFIC REPORTS, v. 10, n. 1, APR 23 2020. (18/20939-3, 13/20549-7, 18/10455-9)

RIBEIRO, ALINE BARBOSA; DA SILVA, THAIS MARQUES; SANTOS-JUNIOR, NILTON NASCIMENTO; CASTANIA, JACI AIRTON; FAZAN, JR., RUBENS; SALGADO, HELIO CESAR. Short-term effect of ligature-induced periodontitis on cardiovascular variability and inflammatory response in spontaneously hypertensive rats. BMC ORAL HEALTH, v. 21, n. 1, OCT 11 2021. (18/20939-3, 18/10455-9, 20/06043-7)