Expression of genes involved in fatty acid profile and post mortem proteolysis of Nellore and Galician Blond x Nellore cattle

• Grant number: 18/01457-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): July 01, 2018
• Effective date (End): August 31, 2019
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Angélica Simone Cravo Pereira
• Grantee: Adrielle Matias Ferrinho
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The goal of this study is to evaluate the levels of genes involved in the fatty acids synthesis, as well as, the levels of proteins involved in the proteolytic process during beef post mortem period of Nellore and crossbred F1 Galician Blond x Nellore, finished in feedlot. Contrasting the meat fatty acid profile these two genetic groups, and the shear force of the aged longissimus muscle. Are being used 16 Nellore bulls and 16 crossbred Galician Blond x Nellore bulls, with an average age of 11 months and 280 kg ± 15 kg of initial live weight. The animals are kept under the same management conditions and it receiving the same ad libitum diet, containing 70% concentrate and 30% forage in dry matter for finishing diet 1 and a 85:15 ratio for finishing diet 2, with corn silage and raw cane bagasse. At the end of experimental period of 120 days, the animals will be harvested and samples of longissimus muscle will be collected for the extraction of RNA and degradation of proteins. During deboning, longissimus muscle samples will be collected for the fatty acid profile analysis and shear force. Samples to shear force and protein degradation will be aging by zero, 7, 14 e 21 days. The genes evaluated will be: PPAR³, SREBP-1c, FABP4, ACC, FAS, SCD, MSTN, CAPN1, CAPN2 e CAST. It is expected that the genetic groups influence the levels of genes involved in lipid metabolism and the fatty acid profile of the beef, as well as a difference in protein expression. The results may suggest which genetic group would be healthier, as regards the profile of fatty acids for human health, besides a beef with better sensorial quality referring to the tenderness attribute. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERRINHO, ADRIELLE M.; DE MOURA, GABRIELLA VESPE; MARTINS, TAIANE DA SILVA; MUNOZ, JULIAN; MUELLER, LENISE F.; GARBOSSA, POLLYANA LEITE MATIOLI; DE AMORIM, TAMYRES RODRIGUES; JESSICA, LUANA GEMELLI; FUZIKAWA, INGRID HARUMI DE SOUZA; PRADO, CIBELE; et al. Rubia Gallega x Nelore crossbred cattle improve beef tenderness through changes in protein abundance and gene expression. LIVESTOCK SCIENCE, v. 251, SEP 2021. (18/01457-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FERRINHO, Adrielle Matias. Expression of genes involved in fatty acid profile and proteolysis post mortem from Nelore and Rubia Gallega x Nelore cattle. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Zootecnica e Engenharia de Alimentos (FZE/BT) Pirassununga.