The effects of concurrent training on the epigenetic mechanism of DNA methylation

• Grant number: 18/16513-0
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: November 01, 2018
• End date: April 30, 2019
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Carlos Ugrinowitsch
• Grantee: Guilherme Defante Telles
• Supervisor: John Alan Hawley
• Host Institution: Escola de Educação Física e Esporte (EEFE). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Australian Catholic University, Melbourne (ACU), Australia
• Associated to the scholarship: 17/01297-8 - Effects of concurrent training on the epigenetic mechanism of DNA methylation, BP.MS

Abstract

The DNA Methylation Level responses in myogenic genes related to satellite cells (SCs) activation can be mechanisms that explain the lower muscle gains with Concurrent Training (CT) compared to Resistance Training (RT) alone, phenomenon known as Interference Effect. Aim: To determine and compare the methylation and the mRNA expression levels of the myogenic regulatory factors (MyoD, Myf5, MyoG, MRF4) and mitochondrial-related genes (PGC-1alpha, TFAM, PPAR-gamma) after Resistance Exercise (RE), Endurance Exercise (EE) and Concurrent Exercise (CE). Methods: 9 untrained young men participated in a randomized and balanced cross over experimental design. Each volunteer performed a bout of RE, EE and CE. The RE was composed by 2 exercises (Leg Press 45 and Leg extension), 2x8-12 maximum repetitions with 60s rest. The EE was performed on a treadmill, composed by 4 minutes warm up at 50% of the velocity reached on the maximum oxygen consumption (vVO2max) followed by 12 x 1 minute sprints at 100% of the vVO2max with 1 minute rest at 50% of vVO2max. The CE was composed by the same RE protocol followed by EE with 5 minutes rest between them. Twelve muscle samples were taken: immediately before, and 3 times after each exercise session (immediately (0h), 4 hours and 8 hours). Our hypothesis is that the methylation level of myogenic gene downregulation with CE will be lower than with RE, which could be one factor to induce the Interference Effect. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TELLES, GUILHERME DEFANTE; LIBARDI, CLEITON AUGUSTO; CONCEICAO, MIGUEL SOARES; VECHIN, FELIPE CASSARO; LIXANDRAO, MANOEL EMILIO; LUGNANI DE ANDRADE, ANDRE LUIS; GUEDES, DANIEL NOVAIS; UGRINOWITSCH, CARLOS; CAMERA, DONNY MICHAEL. Time Course of Skeletal Muscle miRNA Expression after Resistance, High-Intensity Interval, and Concurrent Exercise. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 53, n. 8, p. 1708-1718, AUG 2021. (18/12150-0, 18/16513-0, 17/01297-8)

TELLES, GUILHERME DEFANTE; LIBARDI, CLEITON AUGUSTO; CONCEICAO, MIGUEL SOARES; VECHIN, FELIPE CASSARO; LIXANDRAO, MANOEL EMILIO; ROTEA MANGONE, FLAVIA REGINA; PAVANELLI, ANA CAROLINA; NAGAI, MARIA APARECIDA; CAMERA, DONNY MICHAEL; HAWLEY, JOHN A.; et al. Interrelated but Not Time-Aligned Response in Myogenic Regulatory Factors Demethylation and mRNA Expression after Divergent Exercise Bouts. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 55, n. 2, p. 10-pg., 2023-02-01. (18/12150-0, 18/16513-0, 17/01297-8)

TELLES, GUILHERME DEFANTE; LIXANDRAO, MANOEL; CONCEICAO, MIGUEL. Effects of combined exercise training in older adults: a potential relationship between muscle fibre satellite cell function and capillarization. JOURNAL OF PHYSIOLOGY-LONDON, v. 597, n. 8, p. 2-pg., 2019-04-15. (17/01297-8, 18/16513-0, 16/22635-6, 15/19756-3)