Gene profiling of embryonic stem cells haploinsufficient for STI1 in the maintenance of pluripotent status

• Grant number: 18/19320-9
• Support type: Scholarships in Brazil - Master
• Effective date (Start): January 01, 2019
• Effective date (End): December 31, 2019
• Field of knowledge: Biological Sciences - Biology
• Principal researcher: Marilene Hohmuth Lopes
• Grantee: Camila Felix de Lima Fernandes
• Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Stress Inducible Protein 1 (STI1) is a protein constutively expressed from the early stages of development through adult life, being highly conserved throughout evolutionary history. STI1 acts as a co-chaperone, forming in the cytoplasm a protein complex with the heat shock proteins of 70kDa and 90kDa, aiding in the folding and localization of proteins, contributing to protein homeostasis (proteostasis). In addition to its primary role, studies indicate that STI1 is involved in a number of other important processes. The importance of this protein in the biology of organisms is very evident in experiments performed with mice deficient for STI1, which were not feasible, with few surviving until about the tenth day of intrauterine life. Despite the phenotype of non-viability revealed, the mechanisms that lead to early degeneration of the embryo remain unknown. Thus, the main objective of this work is the transcriptome analysis of murine embryonic stem cell lineages with different levels of STI1 expression, focusing on haploinsufficient lineages, compared to wild-type lineages. The main objective is to analyze the gene expression profile of these cells, observing which genes and signaling pathways stand out, once the protein has been partially depleted. The results of this analysis will contribute to a better understanding of the importance of STI1 for pluripotency in embryonic stem cells and their role in early embryonic development. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

IGLESIA, REBECA PIATNICZKA; DE LIMA FERNANDES, CAMILA FELIX; COELHO, BARBARA PARANHOS; PRADO, MARIANA BRANDAO; MELO ESCOBAR, MARIA ISABEL; DONA RODRIGUES ALMEIDA, GUSTAVO HENRIQUE; LOPES, MARILENE HOHMUTH. Heat Shock Proteins in Glioblastoma Biology: Where Do We Stand?. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 22 NOV 2019. Web of Science Citations: 0.

DE LIMA FERNANDES, CAMILA FELIX; IGLESIA, REBECA PIATNICZKA; MELO-ESCOBAR, MARIA ISABEL; PRADO, MARIANA BRANDAO; LOPES, MARILENE HOHMUTH. Chaperones and Beyond as Key Players in Pluripotency Maintenance. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, v. 7, AUG 2 2019. Web of Science Citations: 1.