Effect of 12-oleic acid ester of hydroxy fatty acid (12-OAHSA) on inflammatory and metabolic pathways of macrophage activation

• Grant number: 18/22505-0
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2019
• Effective date (End): February 28, 2021
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Pedro Manoel Mendes de Moraes Vieira
• Grantee: Ana Campos Codo
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Immune cells are dynamic and respond to foreign antigens and tolerate self-antigens requiring, a process that requires metabolic adaptation for survival, proliferation, and return to homeostasis. Fatty acids regulate several metabolic and signaling pathways, including insulin signaling, calcium flux, and inflammation. Exposure of macrophages to saturated fatty acids or LPS activates pathways that lead to secretion of pro-inflammatory cytokines such as TNF-± and IL-6. On the other hand, unsaturated fatty acids or short-chain fat acids reduce inflammation. Recently, a new class of lipids not previously seen in mammalian tissues has been described. Structural studies described that they are fatty acyl hydroxy fatty acids (FAHFA). When the fatty acid group is oleate and the ester bond is in the position 12, the FAHFA is called oleoyl-12-hydroxystearic acid (12-OAHSA). Members of this class of lipids are present in various tissues and in the serum of humans and mice. Some fatty acids act as signaling molecules that regulate physiological functions, such as insulin secretion, inflammation and hepatic glucose production. Because preliminary data show that treatment with 12-OAHSA reduces TNF-± production in LPS-activated microglia, we hypothesized that this FAHFA acts as an anti-inflammatory lipid in macrophages. Therefore, the hypothesis of this study is that 12-OAHSA acts as an anti-inflammatory molecule in macrophages, which leads to decreased the production of pro-inflorescence cytokines or increase the production of anti-inflammatory cytokines, a process tat may be regulated by metabolic changes in macrophages. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, FELIPE CORREA; AGUIAR, CRISTHIANE; PEREIRA, JESSICA A. S.; MONTEIRO, LAUAR DE BRITO; DAVANZO, GUSTAVO GASTAO; CODO, ANA CAMPOS; DE FREITAS, LEONARDO PIMENTEL; BERTI, ALINE SIQUEIRA; FERRUCCI, DANILO LOPES; CASTELUCCI, BIANCA GAZIERI; et al. Ghrelin effects on mitochondrial fitness modulates macrophage function. Free Radical Biology and Medicine, v. 145, p. 61-66, DEC 2019. (17/23679-0, 16/18031-8, 17/12848-5, 18/22505-0, 15/15626-8, 17/00079-7, 17/06225-5)

FACHI, J. L.; PRAL, L. P.; DOS SANTOS, J. A. C.; CODO, A. C.; DE OLIVEIRA, S.; FELIPE, J. S.; ZAMBOM, F. F. F.; CAMARA, N. O. S.; VIEIRA, P. M. M. M.; COLONNA, M.; et al. Hypoxia enhances ILC3 responses through HIF-1 alpha-dependent mechanism. MUCOSAL IMMUNOLOGY, v. 14, n. 4, JAN 2021. (15/15626-8, 19/11662-0, 18/22505-0, 18/15313-8, 18/02208-1, 17/06577-9, 19/06372-3, 17/16280-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CODO, Ana Campos. Effect of oleoil-12-hydroxystearic acid (12-OAHSA) in the activation of macrophages. 2021. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.