Physicochemical properties, cytotoxicity, and penetration into dentinal tubules of calcium hypochlorite with or without surfactants

• Grant number: 18/24662-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2019
• End date: September 30, 2022
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Gisele Faria
• Grantee: Eric Hernán Coaguila Llerena
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Since the root canal irrigants may contact with the periapical tissues, it should be taken into consideration its possible cytotoxic effects. In addition, the physicochemical properties and the ability to penetrate areas not reached by the instruments are essential for the disinfection of the root canal system. Calcium hypochlorite [Ca(OCl)2] has been suggested as a potential alternative irrigant to sodium hypochlorite (NaOCl). The aim of this study will be to assess the physicochemical properties (part 1), cytotoxicity and effect on the biology of osteoblasts (part 2) and penetration into dentinal tubules (part 3) of Ca(OCl)2 with or without surfactants compared to NaOCl. In part 1, the physicochemical properties of NaOCl and Ca(OCl)2 with or without the surfactants Tween 80 (T80), Triton X-100 (TRX), benzalkonium chloride (BAK) or cetrimide will be evaluated. The pH will be assessed by using digital pH-meter, the surface tension by pendant drop method and the free active chlorine by spectrophotometry. In part 2, L929 fibroblasts or osteoblasts-like Saos2 in culture will be exposed to NaOCl and Ca(OCl)2 in association or not to with surfactants. The cell viability will be evaluated by means of the methyltetrazolium (MTT) and neutral red assays, the cytoskeleton by means of actin labeling, the type of cell death by flow cytometry, the ultrastructure by transmission electron microscopy and the activity of phosphatase alkaline by the calculation of thymolphthalein release. In part 3, single-rooted human teeth will be instrumented, stained with crystal violet and irrigated with NaOCl or Ca(OCl)2 associated or not with surfactants. The penetration into dentin will be evaluated at the cervical, middle and apical thirds. The statistical analysis of the results will be carried out using a significance level of 5%. The statistical tests will be chosen according to the distribution and homoscedasticity of the data. Ultimately, it is our expectation to obtain information to understand more deeply the mechanism of action of Ca(OCl)2 on eukaryotic cells, its physicochemical properties and penetration into dentinal tubules to provide subsidies for its indication or not in the endodontic clinic. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HERNÁN COAGUILA-LLERENA; VICTOR MANUEL OCHOA-RODRIGUEZ; GABRIELA MARIANA CASTRO-NÚÑEZ; GISELE FARIA; JULIANE MARIA GUERREIRO-TANOMARU; MARIO TANOMARU-FILHO. Physicochemical Properties of a Bioceramic Repair Material - BioMTA. Brazilian Dental Journal, v. 31, n. 5, p. 511-515, 2020-11-02. (18/24662-6)

KENNIA SCAPIN VIOLA; HERNÁN COAGUILA-LLERENA; ELISANDRA MARCIA RODRIGUES; CÍNTIA SILVA SANTOS; GISSELLE MORAIMA CHÁVEZ-ANDRADE; MIRIAM GRAZIELE MAGRO; MARIO TANOMARU-FILHO; JULIANE MARIA GUERREIRO-TANOMARU; GISELE FARIA. Different formulations of peracetic acid: effects on smear layer removal, dentine erosion, cytotoxicity and antibiofilm activity. Journal of Applied Oral Science, v. 30, 2022-03-28. (12/18203-2, 18/24662-6, 17/05784-0)

MANUEL PALOMINO-DELGADO; HERNÁN COAGUILA-LLERENA; CARLOS MENDIOLA-AQUINO; GISELE FARIA. Management of unfavorable iatrogenic perforations during post placement using mineral trioxide aggregate: report of two cases. Revista Estomatológica Herediana, v. 31, n. 4, p. 311-316, 2021-10-05. (18/24662-6)

OCHOA-RODRIGUEZ, VICTOR MANUEL; WILCHES-VISBAL, JORGE HOMERO; ROMA, BARBARA; COAGUILA-LLERENA, HERNAN; TANOMARU-FILHO, MARIO; GONCALVES, ANDREA; SPIN-NETO, RUBENS; FARIA, GISELE. Radiopacity of endodontic materials using two models for conversion to millimeters of aluminum. BRAZILIAN ORAL RESEARCH, v. 34, p. 10-pg., 2020-01-01. (18/24662-6)

FERNANDES, CELIO JUNIOR DA COSTA; CASSIANO, ANA FLAVIA BALESTRERO; HENRIQUE-SILVA, FLAVIO; CIRELLI, JONI AUGUSTO; DE SOUZA, EDUARDO PEREIRA; COAGUILA-LLERENA, HERNAN; ZAMBUZZI, WILLIAN FERNANDO; FARIA, GISELE. Recombinant sugarcane cystatin CaneCPI-5 promotes osteogenic differentiation. TISSUE & CELL, v. 83, p. 10-pg., 2023-08-01. (20/09576-6, 16/08888-9, 19/21807-6, 17/05784-0, 18/24662-6)

BALESTRERO CASSIANO, ANA FLAVIA; COAGUILA-LLERENA, HEMAN; SANTOS, CINTIA SILVA; DA SILVA, LUANA RAPHAEL; BAHIA NOGUEIRA, LUCAS FABRICIO; CIANCAGLINI, PIETRO; FARIA, GISELE. The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells. JOURNAL OF ENDODONTICS, v. 48, n. 12, p. 10-pg., 2022-12-01. (18/24662-6)

VICTOR MANUEL OCHOA-RODRÍGUEZ; JORGE HOMERO WILCHES-VISBAL; BARBARA ROMA; HERNÁN COAGUILA-LLERENA; MÁRIO TANOMARU-FILHO; ANDRÉA GONÇALVES; RUBENS SPIN-NETO; GISELE FARIA. Radiopacity of endodontic materials using two models for conversion to millimeters of aluminum. Brazilian Oral Research, v. 34, 2020-07-15. (18/24662-6)

HERNÁN COAGUILA-LLERENA; JULIA DA SILVA TOLEDO; ANA PAULA RAMOS; GISELE FARIA. Physicochemical properties and penetration into dentinal tubules of calcium hypochlorite with surfactants. Brazilian Dental Journal, v. 33, n. 2, p. 1-11, 2022-04-29. (18/24662-6)

COAGUILA-LLERENA, H.; RODRIGUES, E. M.; SANTOS, C. S.; RAMOS, S. G.; MEDEIROS, M. C.; CHAVEZ-ANDRADE, G. M.; GUERREIRO-TANOMARU, J. M.; TANOMARU-FILHO, M.; FARIA, G.. Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells. International Endodontic Journal, v. 53, n. 9, JUL 2020. (18/24662-6)

TEVES, ABEL; BLANCO, DANIEL; CASARETTO, MARIO; TORRES, JOHN; ALVARADO, DEBORA E.; COAGUILA-LLERENA, HERNAN; FARIA, GISELE; JARAMILLO, DAVID E.. Multispecies biofilm removal by XP-endo Finisher and passive ultrasonic irrigation: A scanning electron microscopy study. AUSTRALIAN ENDODONTIC JOURNAL, JUL 2021. (18/24662-6)

HARADA, TAKESHI; HARADA, KAZUMA; NOZOE, AKIRA; TANAKA, SUSUMU; KOGO, MIKIHIKO. Novel Surgical Approach for the Successful Removal of Overextruded Separated Endodontic Instrument. JOURNAL OF ENDODONTICS, v. 47, n. 12, p. 1942-1952, DEC 2021. (18/24662-6)

COAGUILA-LLERENA, HERNAN; LAZO-QUEZADA, GERALDINE; TEVES, ABEL; ZEVALLOS-CHAVEZ, MARCO; FARIA, GISELE. Removal of separated instruments from unfavourable locations: Case reports using the HBW ultrasonic ring or a surgical approach. AUSTRALIAN ENDODONTIC JOURNAL, v. N/A, p. 7-pg., 2022-08-06. (18/24662-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LLERENA, Eric Hernán Coaguila. Root canal irrigation: physicochemical and biological properties of calcium hypochlorite and effects of the GentleWave system in endodontic treatment. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.