Development of a point-of-care biosensor for the swift determination of D-Dimers

• Grant number: 18/13922-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2019
• End date: August 31, 2021
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Thiago Regis Longo Cesar da Paixão
• Grantee: Nikola Tasic
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

D-Dimer (DD) is a general term to describe heterogeneous fibrin degradation products present in blood. Circulating DD values are raised in various clinical conditions although, in general, an elevated DD value indicates some type of thrombosis.It has had particular relevance on the management Pulmonary Embolism (PE). PE is a potentially acute life threating pathology of difficult diagnosis, and it is still a relevant cause of unexpected in-hospital deaths. The creation of low-cost and reliable point-of-care approach for the quantitative bedside determination of DD in clinical samples, thus speeding up the diagnosis of potentially lethal, nonetheless treatable, clinical conditions like PE has the potential to be a game-changer. This project aims to develop a portable electrochemical assay to do precisely that. A collaboration with an international company (Zimmer & Peacock) specialized in bringing new biosensor ideas to life will provide the missing pieces to the university laboratory. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TASIC, NIKOLA; MARTINS, ALISSON BEZERRA; YIFEI, XUE; GOES, MARCIO SOUSA; MARTIN-YERGA, DANIEL; MAO, LANQUN; PAIXAO, THIAGO R. L. C.; GONCALVES, LUIS MOREIRA. Insights into electrochemical behavior in laser-scribed electrochemical paper-based analytical devices. Electrochemistry Communications, v. 121, DEC 2020. (18/20745-4, 17/10522-5, 18/13922-7, 18/14425-7)

MARTINS, ALISSON BEZERRA; LOBATO, ALNILAN; TASIC, NIKOLA; PEREZ-SANZ, FERNANDO J.; VIDINHA, PEDRO; PAIXAO, THIAGO R. L. C.; GONCALVES, LUIS MOREIRA. Laser-pyrolyzed electrochemical paper-based analytical sensor for sulphite analysis. Electrochemistry Communications, v. 107, OCT 2019. (14/50279-4, 18/20745-4, 19/11214-8, 18/13922-7, 17/10522-5, 18/14425-7)

TASIC, NIKOLA; CIRKOVI, JOVANA; RIBIC, VESNA; ZUNIC, MILAN; DAPCEVIC, ALEKSANDRA; BRANKOVIC, GORAN; BRANKOVI, ZORICA. Effects of the silver nanodots on the photocatalytic activity of mixed-phase TiO2. Journal of the American Ceramic Society, v. 105, n. 1, AUG 2021. (18/13922-7)

GROTHE, RENATA A.; LOBATO, ALNILAN; MOUNSSEF, BASSIM; TASI, NIKOLA; BRAGA, ATAUALPA A. C.; MALDANER, ADRIANO O.; ALDOUS, LEIGH; PAIXAO, THIAGO R. L. C.; GONCALVES, LUIS MOREIRA. Electroanalytical profiling of cocaine samples by means of an electropolymerized molecularly imprinted polymer using benzocaine as the template molecule. ANALYST, v. 146, n. 5, p. 1747-1759, MAR 7 2021. (19/05569-8, 18/13922-7, 14/25770-6, 15/01491-3, 18/14425-7, 18/08782-1, 19/11214-8)

TASIC, NIKOLA; CAVALCANTE, LETICIA; DEFFUNE, ELENICE; GOES, MARCIO SOUSA; PAIXAO, THIAGO R. L. C.; GONCALVES, LUIS MOREIRA. Probeless and label-free impedimetric biosensing of D-dimer using gold nanoparticles conjugated with dihexadecylphosphate on screen-printed carbon electrodes. Electrochimica Acta, v. 397, NOV 20 2021. (18/13922-7, 18/08782-1, 17/10522-5, 18/14425-7)

BOSSARD, BRUNA; GROTHE, RENATA ANTUNES; MARTINS, ALISSON BEZERRA; LOBATO, ALNILAN; TASIC, NIKOLA; PAIXAO, THIAGO R. L. C.; GONCALVES, LUIS MOREIRA. Nanographene laser-pyrolyzed paper electrodes for the impedimetric detection of D-glucose via a molecularly imprinted polymer. MONATSHEFTE FUR CHEMIE, v. 153, n. 12, p. 7-pg., 2022-10-14. (18/14425-7, 17/10522-5, 18/13922-7, 18/08782-1, 19/11214-8)

SPOSITO, HEITOR GABRIEL MARTINS; LOBATO, ALNILAN; TASIC, NIKOLA O.; MALDANER, ADRIANO O.; PAIXAO, THIAGO R. L. C.; GONCALVES, LUIS MOREIRA. Swift electrochemical sensing of diltiazem employing highly-selective molecularly-imprinted 3-amino-4-hydroxybenzoic acid. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 911, p. 7-pg., 2022-03-11. (18/13922-7, 18/14425-7, 19/16491-0, 19/11214-8, 17/10522-5, 18/08782-1, 20/04268-1)

TASIC, NIKOLA; PAIXAO, THIAGO R. L. C.; GONCALVES, LUIS MOREIRA. Biosensing of D-dimer, making the transition from the central hospital laboratory to bedside determination. Talanta, v. 207, JAN 15 2020. (17/10522-5, 18/08782-1, 18/13922-7, 18/14425-7)