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Sealing of ramifications located in the apical 1 mm of the root when using two different working lengths: a nanoCT evaluation

Grant number: 18/25051-0
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): May 01, 2019
Effective date (End): October 31, 2019
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal researcher:Alexandre Augusto Zaia
Grantee:Natália Siqueira Lobo
Supervisor abroad: Reinhilde Jacobs
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Research place: University of Leuven (KU Leuven), Belgium  
Associated to the scholarship:17/05391-9 - Incidence of ramifications located in the apical 3 mm of the root with the use of digital periapical radiography, CBCT and computerized microtomography, BP.MS

Abstract

The idea that apical ramifications should be filled for successful endodontic therapy has been accepted by many clinicians and researchers. The high percentage of ramifications in the apical portion of root canals indicates the importance of sealing this region, in order to avoid persistent bacterial contamination. Thus, the purpose of this study is to evaluate the sealing of ramifications located in the apical 1 mm after root canal shaping at different working lengths using Nano-computed tomography (nanoCT) analysis. In this in vitro study, 56 mandibular premolars were scanned with Microcomputed tomography SkyScan 1174 (Bruker, Kontich, Belgium). All images were evaluated by one oral radiologist and one endodontist, which determined the presence or absence of apical ramifications. Twenty-four mandibular pre-molars with apical ramifications were selected and randomly divided into 2 groups (n = 12). The group A will have the root canals shaped 1 mm short of the apical foramen (AF) and the gutta-percha cone and sealer will be limited at the apical stop (1 mm short of the AF). The group B will have the root canals shaped throughout their entire extent with foramen enlargement. On group B the gutta-percha cone will stop 1 mm short of the AF, by friction within the dentin walls, and the sealer will fill the last millimeter of the root canal. The sealing of ramifications will be evaluated through the comparison of nanoCT images obtained before instrumentation and after filling. The imagens will be evaluated in consensus by 2 observers to verify the presence/absence of sealing in apical ramifications. Fisher's exact test will be applied to compared both working lengths with significance level of 5% (±=0,05). A descriptive analysis of the obtained data will be performed as well. The results of this study may provide information about the ability of the sealer to penetrate inside the apical ramifications and guarantee better rate of success in the endodontic therapy. (AU)

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