Resistome and mobilome in genomes of bacteria from diverse Brazilian regions

• Grant number: 19/00390-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2019
• End date: April 30, 2021
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: María Eugenia Guazzaroni
• Grantee: Tiago Cabral Borelli
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 15/04309-1 - Novel approaches to improve functional screening of biocatalysts in metagenomic libraries, AP.JP

Abstract

The benefits of the antibiotics are unquestionable. The usage of this drugs allowed to cure bacterial infections and gave safer conditions to patients who are through immunologically debilitant treatment as chemotherapy and organ transplantations. However, the growing usage both in health clinic and livestock/fish farming (among other) caused the fast evolution of antibiotic resistance genes due to its adaptative and competitive advantages, resulting in a sanitary crisis. Additionally, many antibiotic resistance genes are clustered to mobile genetic elements allowing its spread through horizontal gene transference between bacteria. And due to this antibiotic crisis, there will be 10^6 deaths annually by antibiotic resistant bacteria in 2050. Thus, the study of antibiotic resistant bacteria became a rich research field, and due to new generation sequencing (NGS) and metagenomic techniques a huge amount of data has been accumulated all over the world (10^15 byes). Therefore, there is a need to create an integrative approach which can analyze those data and draw significative relations both at genomic and metagenomic levels. In this way, the current project aims to use bioinformatic techniques and web science tools to map the resistome and mobilome of bacterial genomes from Brazilian biomes. Still, we intent to better understand the national panorama of antibiotic resistance genes by co-occurrence webs to understand ecological and evolutive relations involving resistant bacteria as well as to comprehend the interaction of different resistance determinants and how they spread through pathogenic and non-pathogenic bacteria. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIBEIRO, LUCAS FERREIRA; AMARELLE, VANESA; ALVES, LUANA DE FATIMA; VIANA DE SIQUEIRA, GUILHERME MARCELINO; LOVATE, GABRIEL LENCIONI; BORELLI, TIAGO CABRAL; GUAZZARONI, MARIA-EUGENIA. Genetically Engineered Proteins to Improve Biomass Conversion: New Advances and Challenges for Tailoring Biocatalysts. Molecules, v. 24, n. 16, AUG 2019. (16/18827-7, 15/04309-1, 18/18158-3, 18/07261-8, 19/00390-0, 16/06323-4)

BORELLI, TIAGO CABRAL; LOVATE, GABRIEL LENCIONI; SCARANELLO, ANA FLAVIA TONELLI; RIBEIRO, LUCAS FERREIRA; ZARAMELA, LIVIA; PEREIRA-DOS-SANTOS, FELIPE MARCELO; SILVA-ROCHA, RAFAEL; GUAZZARONI, MARIA-EUGENIA. Combining Functional Genomics and Whole-Genome Sequencing to Detect Antibiotic Resistance Genes in Bacterial Strains Co-Occurring Simultaneously in a Brazilian Hospital. ANTIBIOTICS-BASEL, v. 10, n. 4, APR 2021. (19/00390-0, 15/04309-1, 19/06672-7, 16/18827-7, 19/15675-0, 18/18158-3)