Photodynamic inactivation of Bacterial Pneumonia with nebulization of the photosensitizer and extracorporeal illumination

• Grant number: 18/18188-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2019
• End date: June 30, 2022
• Field of knowledge: Biological Sciences - Biophysics - Radiology and Photobiology
• Principal Investigator: Vanderlei Salvador Bagnato
• Grantee: Giulia Kassab
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID

Abstract

This project aims to solve, in an unconventional matter, the problem of pulmonary infections that do not respond to antibiotics. Pneumonia is one of the largest causes of death worldwide, and it is mainly caused by bacteria. Due to the difficulty in the development and approval of new antibiotics, and the speed in which resistant strains appear, the search for treatments that are effective, safe, and to which there is no resistance becomes urgent. The Photodynamic Inactivation (PDI) of microorganisms presents itself as an excellent alternative, since there has not been any description of development of resistance to it since its discovery in 1900. Over the past 5 years, our research group has studied the applicability of PDI in the treatment of bacterial Pneumonia, using the photosensitizer Indocyanine Green (ICG) and extracorporeal activation with infrared light. So far, we have demonstrated the principle in vitro and in a murine model, established a pulmonary delivery method for ICV that is suitable for clinical use, and showed the extracorporeal delivery of light in bigger animals. This project proposes to give the next steps required for the protocol to become a clinical treatment. That includes the optimization of the parameters in the murine model, the validation in the porcine model and, if possible, the first clinical case. This research shall have great scientific and social impact. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KASSAB, GIULIA; CHEBURKANOV, VSEVOLOD; WILLIS, JACE; MOULE, MADELEINE G.; KURACHI, CRISTINA; YAKOVLEV, VLADISLAV; CIRILLO, JEFFREY D.; BAGNATO, VANDERLEI S.. Safety and delivery efficiency of a photodynamic treatment of the lungs using indocyanine green and extracorporeal near infrared illumination. Journal of Biophotonics, AUG 2020. (18/18188-0, 13/07276-1)

DIAZ TOVAR, JOHAN SEBASTIAN; KASSAB, GIULIA; BUZZA, HILDE HARB; BAGNATO, VANDERLEI SALVADOR; KURACHI, CRISTINA. hotodynamic inactivation of Streptococcus pneumoniae with external illumination at 808 nm through the ex vivo porcine thoracic cag. Journal of Biophotonics, v. 15, n. 2, DEC 2021. (13/07276-1, 16/14033-6, 14/50857-8, 18/18188-0)

WILLIS, JACE A.; CHEBURKANOV, VSEVOLOD; KASSAB, GIULIA; SOARES, JENNIFER M.; BLANCO, KATE C.; BAGNATO, VANDERLEI S.; YAKOVLEV, VLADISLAV V.. Photodynamic viral inactivation: Recent advances and potential applications. APPLIED PHYSICS REVIEWS, v. 8, n. 2, JUN 2021. (18/18188-0, 13/07276-1, 19/13569-8)

WILLIS, JACE A.; CHEBURKANOV, VSEVOLOD; CHEN, SHAORONG; SOARES, JENNIFER M.; KASSAB, GIULIA; BLANCO, KATE C.; BAGNATO, VANDERLEI S.; DE FIGUEIREDO, PAUL; YAKOVLEV, VLADISLAV V.. Breaking down antibiotic resistance in methicillin-resistant Staphylococcus aureus: Combining antimicrobial photodynamic and antibiotic treatments. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v. 119, n. 36, p. 8-pg., 2022-09-06. (13/07276-1, 18/18188-0, 21/09952-0)

KASSAB, GIULIA; DIAZ TOVAR, JOHAN SEBASTIAN; PEREIRA SOUZA, LUCAS MIGUEL; MAGALHAES COSTA, RAYLA KELLY; SILVA, RUDIELSON SANTOS; PIMENTEL, ANDRE SILVA; KURACHI, CRISTINA; BAGNATO, VANDERLEI SALVADOR. Lung surfactant negatively affects the photodynamic inactivation of bacteria-in vitro and molecular dynamic simulation analyses. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v. 119, n. 25, p. 9-pg., 2022-06-21. (18/18188-0, 14/50857-8, 13/07276-1, 14/50983-3)

TOVAR, JOHAN SEBASTIAN DIAZ; KASSAB, GIULIA; INADA, NATALIA MAYUMI; BAGNATO, VANDERLEI SALVADOR; KURACHI, CRISTINA. Photobleaching Kinetics and Effect of Solvent in the Photophysical Properties of Indocyanine Green for Photodynamic Therapy. ChemPhysChem, v. 24, n. 18, p. 10-pg., 2023-08-13. (18/18188-0, 13/07276-1, 14/50857-8)

BUZZA, HILDE HARB; ALVES, FERNANDA; TOME, ANA JULIA BARBOSA; CHEN, JUAN; KASSAB, GIULIA; BU, JIACHUAN; BAGNATO, VANDERLEI SALVADOR; ZHENG, GANG; KURACHI, CRISTINA. Porphyrin nanoemulsion for antimicrobial photodynamic therapy: effective delivery to inactivate biofilm-related infections. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v. 119, n. 46, p. 9-pg., 2022-11-15. (21/01324-0, 18/18188-0, 16/14033-6, 13/07276-1, 14/50857-8)

DIAZ TOVAR, JOHAN SEBASTIAN; KASSAB, GIULIA; INADA, NATALIA MAYUMI; BAGNATO, VANDERLEI SALVADOR; KURACHI, CRISTINA; IEEE. Photodegradation in the infrared region of indocyanine green in aqueous solution. 2019 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE (SBFOTON IOPC), v. N/A, p. 5-pg., 2019-01-01. (18/18188-0)

FEITOR, JESSICA F.; BRAZACA, LAIS C.; LIMA, AMANDA M.; FERREIRA, VINICIUS G.; KASSAB, GIULIA; BAGNATO, VANDERLEI S.; CARRILHO, EMANUEL; CARDOSO, DANIEL R.. Organ-on-a-Chip for Drug Screening: A Bright Future for Sustainability? A Critical Review. ACS BIOMATERIALS SCIENCE & ENGINEERING, v. 9, n. 5, p. 15-pg., 2023-04-04. (17/01189-0, 18/19750-3, 18/18188-0)

KASSAB, GIULIA; CHEBURKANOV, VSEVOLOD; WILLIS, JACE; MOULE, MADELEINE G.; KURACHI, CRISTINA; YAKOVLEV, VLADISLAV; CIRILLO, JEFFREY D.; BAGNATO, VANDERLEI S.. Safety and delivery efficiency of a photodynamic treatment of the lungs using indocyanine green and extracorporeal near infrared illumination. Journal of Biophotonics, v. 13, n. 10, p. 13-pg., 2020-08-12. (18/18188-0, 13/07276-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

KASSAB, Giulia. Pulmonary antimicrobial photodynamic therapy and the role of the lung surfactant. 2022. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.