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Determination of the excretion of artificial sweeteners in saliva and its relation with the secretion of insulin

Grant number: 19/04666-0
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2019
Effective date (End): May 31, 2021
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal Investigator:Paula Midori Castelo Ferrua
Grantee:Carolina Martins Finassi
Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil


According to official data (IBGE, 2011), consumption of diet-type soft drinks is more frequent in the adult Brazilian population. Studies have shown that sweeteners may have potential adverse effects and are not related to a significant weight loss. So far, it is not known how much these products are excreted in saliva and thus may re-stimulate taste buds being swallowed again, remaining in the digestive tract and in the bloodstream for longer. Thus, this study aims to quantify the excretion of artificial sweeteners added to Coca-cola soft drink - sodium cyclamate, acesulfame potassium and aspartame - in saliva and its relation with salivary insulin, amylase and total protein secretion after the ingestion of sweetened beverages. This is a randomized crossover trial. Ten adults (20-25 years old, healthy, 5 men and 5 women, age-paired) will be selected to participate in5blinded sessions/experiments of beverage intake: diet Coca-Cola, Coca-Cola (sucrose), water+sweeteners (cyclamate, acesulfame and aspartame), water+sucrose and water (blanc), randomly chosen, totaling 50 trials. The collection of five saliva samples of the parotid and sublingual glands will be performed in fasting: baseline (T0) and, after drinking the test-drink: T1 (15 minutes after), T2 (30 min), T3 (60 min) and T4 (120 min) for the measurement of the concentration of sodium cyclamate, acesulfame potassium and aspartame (by high performance liquid chromatography-HPLC), insulin (electrochemiluminescence), amylase and total protein (automated enzymatic technique). A pilot study was carried out by the student in the laboratory, and it was possible to observe sodium cyclamate excretion in saliva after an ingestion of diet Coca-Cola on T0, T1, T2, and T3 collections times by means of HPLC, as well the detection of insulin in individual samples (electrochemiluminescence).Data will be analyzed using exploratory statistics, Two-way ANOVA mixed model (sex and test-drink as factors) and matrix of correlation, considering alpha = 5%.

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