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Effect of surface properties of different Ti-6Al-4V discs on bacterial adhesion

Grant number: 19/14287-6
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2019
Effective date (End): October 31, 2020
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Mariana Lima da Costa Valente
Grantee:Gustavo Lopes Puls
Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


The development of scientific researches as well as physical and biological properties of dental implants have become a special treatment modality - as they are one of the main choices for dental loss substitution. However certain types of bacteria present high affinity for metalic surfaces and carry out an important role in the development of peri-implantitis, frequently associated with the failure of implants. This research aims to evaluate the influence of four titanium surfaces (Ti-6Al-4V) in the bacterial adhesion, as well as to establish a correlation between its surface roughness properties and free surface energy. There will be made 40 titanium discs Ti-6Al-4V (Ø 8mm × 3mm), 30 by conventional turned/machined method and 10 by additive manufacture with selective laser fusion method (SLM), divided in 4 analysis groups: G1 - Machined and polished; G2- Machined with H3PO4 + NaOH surface treatment; G3 - Machined with Hidroxiapatitis (HA) surface treatment and G4 - Manufactured by SLM technique. The surface roughness will be evaluated by a 3D laser confocal microscope through medium roughness parameters (Ra, Rz and As) and the free surface energy through static contact angle by using three liquids with different polarities (Diiodometano, Distilled Water, Formamida). To evaluate the formation of microorganisms, the Colony Forming Unit Count (UFC) method will be performed. After collecting data, and according to its distribution, parametric or nonparametric tests will be used, with significance level of 5%.

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