Determination of adhesion efficiency of A. actinomycetemcomitans, S. gordonii and S. oralis on the acquired pellicle formed with saliva from patients with localized aggressive periodontitis and controls with family related and not related

• Grant number: 19/16164-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2019
• End date: January 31, 2021
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Marcia Pinto Alves Mayer
• Grantee: Nicole Stephanie Piteri Porto
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 15/18273-9 - New strategies for the control of periodontitis, AP.TEM

Abstract

The formation of the dental biofilm begins by the adhesion of first colonizers species on a conditioning film adsorbed to the surface of the tooth, called acquired pellicle (APE), followed by proliferation and aggregation to the organisms initially adhered to. Data from our group showed that the differences in oral microbiome among patients with localized aggressive periodontitis (PAgL) and controls reside not only in higher relative abundance of pathogens such as A. actinomycetemcomitans, but also in the reduction of relative abundance of early colonizing organisms. Proteomic assays revealed that the APE of subjects with PAgL had undetectable levels of certain proteins involved in the immune response and antimicrobial activity, differing from the APE of the controls. Aiming to test the hypothesis that the APE composition could interfere in the microbial colonization, this study aims to determine the affinity of A. actinomycetemcomitans, S. gordonii and S. oralis to APE formed with saliva of patients with PAgL and compare to APE formed with saliva from healthy control patients, with a familiar relation to PAgL not related. This analysis will enable a better understanding of involved mechanisms in aggressive periodontitis susceptibility, and could support the development of new strategies of bioengineering of acquired pellicle to favor the dental surface colonization of the by beneficial species.