Identification of Yellow Fever and Chikungunya in mosquitoes captured in the state of Sao Paulo

• Grant number: 19/21568-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2019
• End date: May 12, 2021
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Agreement: MRC, UKRI ; Newton Fund, with FAPESP as a partner institution in Brazil
• Principal Investigator: Ester Cerdeira Sabino
• Grantee: Mariana Cardoso de Pinho
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/14389-0 - Brazil-UK Centre for Arbovirus Discovery, Diagnosis, Genomics and Epidemiology (CADDE), AP.TEM

Abstract

The state of São Paulo has several points that meet the conditions necessary for the transmission of arboviruses: they have vertebrates that can act as reservoir hosts for such viruses, mosquito species known as vectors and humans, subject to infections by such viruses. Thus, the objective of the present work will be to identify the presence of Flavivirus and Alphavirus in mosquitoes (Diptera: Culicidae) collected in São Paulo State. The traps used will be: vacuum cleaner, Shannon and CDC-CO2. Mosquitoes will be transported to the laboratory on dry ice, identified on a cold table and grouped into pools of up to 10 non-engorged females, by species, date and place of collection. After this, the samples will be subjected to Flavivirus detection and Alphavirus by polymerase chain reaction (PCR). Positive samples will be sent for sequencing and phylogenetic analysis. Thus, it is intended to contribute to the knowledge about the existence, distribution and frequency of Flavivirus and Alphavirus in São Paulo State mosquitoes and to raise epidemiological data to help prevent arbovirus transmission to humans. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FARIA, NUNO R.; MELLAN, THOMAS A.; WHITTAKER, CHARLES; CLARO, INGRA M.; CANDIDO, DARLAN DA S.; MISHRA, SWAPNIL; CRISPIM, MYUKI A. E.; SALES, FLAVIA C.; HAWRYLUK, IWONA; MCCRONE, JOHN T.; et al. Genomics and epidemiology of the P.1 SARS-CoV-2 lineage in Manaus, Brazil. Science, v. 372, n. 6544, p. 815+, MAY 21 2021. (20/04558-0, 19/21568-1, 19/21301-5, 18/14389-0, 17/13981-0, 20/04272-9, 18/25468-9, 16/18445-7, 19/21858-0, 18/17176-8, 19/12000-1, 19/07544-2, 18/12579-7, 19/24251-9)