Trophic transfer assessment of the antifouling biocide DCOIT, in nano and non-nanostructured forms, in marine organisms

• Grant number: 19/19898-3
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: December 30, 2019
• End date: December 29, 2020
• Field of knowledge: Physical Sciences and Mathematics - Oceanography - Biological Oceanography
• Principal Investigator: Denis Moledo de Souza Abessa
• Grantee: Bruno Galvão de Campos
• Supervisor: Roberto Carlos Domingues Martins
• Host Institution: Instituto de Biociências (IB-CLP). Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil
• Institution abroad: Universidade de Aveiro (UA), Portugal
• Associated to the scholarship: 17/10211-0 - Ecotoxicological study of the anti-fouling biocide Sea-Nine in neotropical marine invertebrates., BP.DR

Abstract

Using an innovative approach based on nanotechnology, the biocide DCOIT was encapsulated in silica nanoparticles (SiNC) in order to reduce its environmental impact and to improve its antifouling efficacy. However, studies on its bioaccumulation and trophic transference are required, as well as those regarding its toxicity. The present study aims to assess the bioaccumulation and trophic transferability of both free DCOIT and its encapsulated form (SiNC-DCOIT) by feeding bivalves with contaminated algae. Firstly, an experiment will be set up to identify whether microalgae and bivalves can internalize SiNC and bioaccumulate DCOIT. In such experiment, algae and bivalves will be exposed (separately) to a sublethal concentration of DCOIT and SiNC (labeled with a chromophore). Then, by fluorescence microscopy, the internalization of SiNC will be verified. To confirm bioaccumulation, we will develop and refine methods of extraction and quantification of DCOIT in biological matrices (algae and bivalves) and water. Once the bioaccumulation is confirmed and the quantification protocols have already been developed, a second experiment will be set up to verify the trophic transfer of DCOIT and SiNC-DCOIT in bivalves. For this experiment the mussel Mytilus galloprovincialis will be fed with the microalgae Tetraselmis chuii contaminated for 72 h, comprising 4 treatments, algae contaminated with DCOIT or SiNC-DCOIT or SiNC and algae not contaminated. After the feeding and exposure period, the concentrations of DCOIT and SiNC-DCOIT in organisms and water will be quantified to determine if there is trophic transfer. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE CAMPOS, BRUNO GALVAO; DO PRADO E SILVA, MARIANA BRUNI MARQUES; AVELELAS, FRANCISCO; MAIA, FREDERICO; LOUREIRO, SUSANA; PERINA, FERNANDO; DE SOUZA ABESSA, DENIS MOLEDO; MARTINS, ROBERTO. Toxicity of innovative antifouling additives on an early life stage of the oyster Crassostrea gigas: short- and long-term exposure effects. Environmental Science and Pollution Research, JAN 2022. (20/03004-0, 19/19898-3, 18/25379-6, 17/10211-0, 19/24945-0)

NICOLAU DOS SANTOS, JULIANA VITORIA; MARTINS, ROBERTO; FONTES, MAYANA KAROLINE; DE CAMPOS, BRUNO GALVAO; SILVA, MARIANA BRUNI MARQUES DO PRADO E.; MAIA, FREDERICO; DE SOUZA ABESSA, DENIS MOLEDO; PERINA, FERNANDO CESAR. Can Encapsulation of the Biocide DCOIT Affect the Anti-Fouling Efficacy and Toxicity on Tropical Bivalves?. APPLIED SCIENCES-BASEL, v. 10, n. 23, DEC 2020. (18/25379-6, 19/19898-3, 17/10211-0, 16/24033-3)

DE CAMPOS, BRUNO GALVAO; FIGUEIREDO, JOANA; PERINA, FERNANDO; ABESSA, DENIS MOLEDO DE SOUZA; LOUREIRO, SUSANA; MARTINS, ROBERTO. Occurrence, effects and environmental risk of antifouling biocides (EU PT21): Are marine ecosystems threatened?. CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY, v. 52, n. 18, p. 32-pg., 2021-03-29. (17/10211-0, 19/19898-3)