Procedures for establishing experimental organ donor models

• Grant number: 19/19549-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2019
• End date: November 30, 2020
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Paulo Manuel Pêgo-Fernandes
• Grantee: Nathaly Christinie Ikeoka Eroles
• Host Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Experimental models are important tools for understanding physiological and etiopathogenic phenomena. Different animal models are used to study the elements involved in the pathophysiology of post-transplant Primary Graft Dysfunction in order to evaluate new treatments. Currently, we can divide experimental models of lung donors into two major groups: postmortem brain donation and circulatory death donation. In addition, the deleterious effects of hemorrhagic shock can be considered. In this study, we aim to describe and compare the inflammatory process and histopathological disorders related to three lung donor models: donation after brain death; donation after circulatory death and hemorrhagic shock. Nine rats will be divided into three groups: Hemorrhagic Shock (n = 3); Brain death (n = 3); Circulatory death (n = 3). For each of these groups, the procedural contents and hemodynamic analyzes will be described; in addition to respiratory parameters, blood gas analysis, arterial, electrolytes, and lactate. (AU)