Effect of synbiotic ice cream consumption for probiotic strains delivery, short-chain fatty acid production and fecal pH of military in field training

• Grant number: 19/23680-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2020
• End date: May 31, 2021
• Field of knowledge: Health Sciences - Nutrition
• Principal Investigator: Adriane Elisabete Antunes de Moraes
• Grantee: Isabel de Almeida Vieira
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil

Abstract

Probiotics have been indicated as health promoters because they act in the prevention and treatment of acute and chronic health conditions and may improve innate and acquired immunity through various mechanisms. These microorganisms have been recognized as potentially reducing agents of respiratory infections and the incidence of acute infectious diarrhea, and may be indicated for military, especially in simulated acute survival activities, as they are situations of high stress, sleep deprivation and excessive physical activity. weaken the immune system and increase susceptibility to the aforementioned health problems. The aim of this project is to evaluate the probiotics delivery, amount of short-chain fatty acids, ammonium ions production and pH of stools of military supplemented with symbiotic ice cream compared to the placebo group, in order to prospect the activity of probiotics in promoting intestinal and breath health benefits among military personnel in field training situations. The present proposal complements ongoing project analyzes whose objectives included the 30-day supplementation of 80 military men and women with symbiotic ice cream or placebo. From the collected feces, a microbiota sequencing analysis was performed and this Scientific Initiation project proposes to aggregate the pH (pH meter reading), short chain fatty acids (SCFA) (gas chromatography) and ammonia (ammonium ion meter) analyzes of the stool samples collected in the baseline period (after washout), after 30 days of supplementation and after 5 days of field training activity. Additionally, real-time PCR analysis will be performed to quantify supplemented species in the stools (B. animalis and L. acidophilus).The variables will be submitted to two-way ANOVA analysis, followed by Tukey's multiple comparisons test, with a significance level of 5% (p <0.05).