Selection and assessment of the combination between waste of tropical fruits and probiotic strains in the modulation of intestinal microbiota of obese compared to the normal weight using the simulator of the human intestinal microbial ecosystem

• Grant number: 15/13965-0
• Support Opportunities: Regular Research Grants
• Duration: September 01, 2015 - August 31, 2017
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Katia Sivieri
• Grantee: Katia Sivieri
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: Maria Bernadete Amâncio Varesche ; Raquel Bedani Salvio ; Susana Marta Isay Saad

Abstract

The obesity has been considered, nowadays, one of the most frequent pathologies and, its etiology and treatment is related to lifestyle and eating habits. Prebiotics and bioactive compounds, present in tropical fruits wastes, have the potential to promote the modulation of intestinal microbiota, providing many benefits to the host's health, including the reduction obesity risk. On the other hand, the consumption of probiotics in synbiotic associations have demonstrated the beneficial effect on gut health, assisting the intestinal microbiota homeostasis. The aim of this work is to select a synbiotic combination of certain waste of tropical fruits with some probiotic strains, which positively influence the metabolism and the population of normal and obese microbiota, using a Simulator of Human Intestinal Microbial Ecosystem (SHIME). This project will be carried out in three steps. Step 1: It will be performed the viability analysis of three probiotic strains (L. paracasei 431, Bifidobacterium longum BB46 and L. acidophilus LA5) in combination with two waste of tropical fruits (camu-camu and acerola) and commercial pectin using an in vitro system (in batch). The combinations with the best viability, will have it's action, under the intestinal microbiota of normal individuals, tested in SEMH (Step 2). Step 3: The synbiotic combination with the best result in step 2, will have it's action, in the intestinal microbiota of obese individuals, tested in the SHIME. In this step, in addition to the same analysis performed in the second stage of study (ammonia, short chain fatty acids and microbiology dependent cultivation) it will be also realized, both in obese and eutrophic microbiota (control), analysis of the gut microbial diversity, using no dependent methods of cultivation: PCR and sequencing mass rRNA 16S gene using the Illumina HiSeq® platform on each trial. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BIANCHI, FERNANDA; LARSEN, NADJA; TIEGHI, THATIANA DE MELLO; ADORNO, MARIA ANGELA T.; SAAD, I, SUSANA M.; JESPERSEN, LENE; SIVIERI, KATIA. In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin. Food Research International, v. 120, p. 595-602, JUN 2019. (15/13965-0, 15/08228-6, 13/50506-8, 16/20336-1)

BIANCHI, FERNANDA; LARSEN, NADJA; TIEGHI, THATIANA DE MELLO; TALLARICO ADORNO, MARIA ANGELA; KOT, WITOLD; ISAY SAAD, SUSANA MARTA; JESPERSEN, LENE; SIVIERI, KATIA. Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with Bifidobacterium longum BB-46. Applied Microbiology and Biotechnology, v. 102, n. 20, p. 8827-8840, OCT 2018. (15/13965-0, 16/20336-1, 13/50506-8, 15/08228-6)

BIANCHI, FERNANDA; LOPES, NATALIA PONTIN; TALLARICO ADORNO, MARIA ANGELA; SAKAMOTO, ISABEL KIMIKO; GENOVESE, MARIA INES; ISAY SAAD, SUSANA MARTA; SIVIERI, KATIA. Impact of combining acerola by-product with a probiotic strain on a gut microbiome model. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, v. 70, n. 2, p. 182-194, FEB 17 2019. (15/13965-0, 13/50506-8, 15/08228-6)

BIANCHI, FERNANDA; ROCHA FARIA DUQUE, ANA LUIZA; ISAY SAAD, SUSANA MARTA; SIVIERI, KATIA. Gut microbiome approaches to treat obesity in humans. Applied Microbiology and Biotechnology, v. 103, n. 3, p. 1081-1094, FEB 2019. (15/13965-0, 13/50506-8, 15/08228-6)

BIANCHI, FERNANDA; LARSEN, NADJA; TIEGHI, THATIANA DE MELLO; ADORNO, MARIA ANGELA T.; SAAD, SUSANA M., I; JESPERSEN, LENE; SIVIERI, KATIA. In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin. Food Research International, v. 120, p. 8-pg., 2019-06-01. (16/20336-1, 13/50506-8, 15/13965-0, 15/08228-6)