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Differential gene expression of Melipona scutellaris Malpighi tubule transcriptomes exposed to thiamethoxam

Grant number: 20/03527-3
Support type:Scholarships in Brazil - Master
Effective date (Start): September 01, 2020
Effective date (End): March 31, 2022
Field of knowledge:Biological Sciences - Zoology - Applied Zoology
Principal researcher:Osmar Malaspina
Grantee:Lucas Miotelo
Home Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
Associated research grant:17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use, AP.TEM

Abstract

In Brazil, the consumption of pesticides is intensified year after year, with emphasis on the use of the neonicotinoid class. Thiamethoxam, belong to this class, is used in the main Brazilian crops and the indiscriminate use of this compound affects non-target insects, such as bees. The current risk assessment for pollinators uses species of the Apis genus as a model, however, concern about species of stingless bees has increased in recent years. In this sense, Melipona scutellaris has been used as a new model for studies of the toxicity of pesticides on stingless bees. However, up to date, there are no molecular data to elucidate the effects of thiamethoxam on M. scutellaris. Thus, the present project aims to carry out a differential gene expression analysis of the Malpighi tubule transcriptome, for this purpose, foragers bees of M. scutellaris will be exposed to the sublethal concentration of thiamethoxam (CL50/100). Then, the total RNA will be extracted from the organs exposed after one and eight days to thiamethoxam and sent for large-scale sequencing Illumina - RNA-Seq. The reads obtained will be pre-processed and the genes differentially expressed in the treatment conditions will be identified, characterizing this change and relating these results to the metabolic pathways used by the bee for detoxification. This approach will contribute to the discovery of new genes and possible metabolic routes used by M. scutellaris when exposed to thiamethoxam. (AU)

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