Role of ³´ T cells in adaptations of intestinal epithelium induced by inulin diet

• Grant number: 20/00311-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: October 01, 2020
• End date: October 01, 2025
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Marco Aurélio Ramirez Vinolo
• Grantee: Helder Carvalho de Assis
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 18/15313-8 - Investigation of the molecular mechanisms involved in the interaction between microbiota-derived metabolites and host cells during inflammation, AP.JP2
• Associated scholarship(s): 23/02507-7 - Effect of inulin diet on migration and function of intestinal gamma-delta T cells, BE.EP.DD

Abstract

The balance between intestinal microbiota activity and its interactions with immune system is essential for maintenance of homeostasis and prevention of inflammatory diseases. The diet compositions are able to modulate microorganism's populations of microbiota and consequently its activity, through metabolites production that are recognized by epithelial and immune cells. Fermentation of fibers is the main energetical source of microbiota and inulin is a prebiotic soluble fiber widely studied. Many works showed that inulin in able to modulate immune system, preventing infections and metabolic disorders. Inulin promotes IL-22 production, an important cytokine in gut epithelium's health maintenance, however, the action mechanisms involved are not completely elucidated. A work developed by our group showed that inulin-enriched diet promotes remodeling of gut epithelium and proliferation of intestinal stem cells, through IL-22 signaling and its action on epithelium depends on ³´ T cells. The ³´ T cells are present in intestinal epithelium and lamina propria, are sources of IL17 and IL22, are fundamental in maintenance of gut homeostasis and defense against pathogens and their activities are directly associated with modulations promoted by gut microbiota. Knowing the biological relevance of gut microbiota and diet compositions in regulation of immune system and intestinal epithelium, our objective is to investigate how ³´ T cells participate in remodeling of gut epithelium by inulin diet. To achieve that, we will investigate aspects such as migration of ³´ T cells between intestinal compartments, how they interact with inulin-enriched microbiota, which subtypes are involved in gut remodeling and how microenvironment induced by inulin respond to inflammatory stimuli like infections and colorectal cancer. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORREA, RENAN OLIVEIRA; CASTRO, POLLYANA RIBEIRO; FACHI, JOSE LUIS; NIRELLO, VINICIUS DIAS; EL-SAHHAR, SALMA; IMADA, SHINYA; PEREIRA, GABRIEL VASCONCELOS; PRAL, LAIS PASSARIELLO; PEREIRA ARAUJO, NATHALIA VITORIA; FERNANDES, MARIANE FONT; et al. Inulin diet uncovers complex diet-microbiota-immune cell interactions remodeling the gut epithelium. MICROBIOME, v. 11, n. 1, p. 25-pg., 2023-04-26. (16/23142-3, 21/06572-2, 19/06372-3, 19/16113-5, 19/02640-3, 18/15313-8, 20/02919-5, 17/16280-3, 20/14071-0, 21/00393-9, 17/06577-9, 20/13689-0, 21/05269-4, 20/02312-3, 18/10165-0, 20/00311-0)