Validation of target proteins associated with the progression of potentially malignant disorders into Oral Cancer

• Grant number: 19/09692-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: January 01, 2021
• End date: May 31, 2023
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Adriana Franco Paes Leme
• Grantee: Ana Gabriela Costa Normando
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated research grant: 18/18496-6 - The role of alcohol treated-extracellular vesicles in oral cells transformation, AP.TEM

Abstract

Oral Cancer accounts for 2.0% of all new Cancer cases and approximately 90% are Squamous Cell Carcinomas (SCC). The main risk factors for oral SCC are excessive tobacco and alcohol consumption, leading to a complex carcinogenic process. Oral SCC often evolves from potentially malignant disorders such as Oral Leukoplakia (OL), which is one of the most prevalent, and Proliferative Verrucous Leukoplakia (PVL), which is characterized by high rates of malignant transformation and recurrence. OL occurs mainly in men, smokers and alcohol users, while PVL is characterized by female preference with no direct correlation with smoking and alcoholism. Due to the high prevalence of OL and to the high degree of PVL aggressiveness, the search for possible biomarkers would aid in an early diagnosis of the lesions, as well as assist in predicting the risk of malignant transformation. A recent unpublished study conducted in the group analyzed saliva samples from 58 patients with active lesions of OL, PVL, SCC, and control by discovery-based proteomics and identified 41 proteins differentially expressed between the groups. Thus, the objective of the present project is to continue this study through the verification of common proteins between lesions of OL, PVL, and SCC in saliva and Extracellular Vesicles (EVs) of biopsies, which will be analyzed by target-based proteomics, as well as the biopsy samples will be evaluated by immunohistochemistry. At the end of the study, it is expected to have verified salivary, EVs, and tissue proteins to be used in clinical practice in the future, assisting in the early diagnosis of lesions and in the indication of the risk of malignancy of OL and PVL lesions, improving the prognosis of such patients. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, ERISON SANTANA; NORMANDO, ANA GABRIELA COSTA; SCARINI, JOAO FIGUEIRA; CRESCENCIO, LIVIA RAMALHO; DE LIMA-SOUZA, REYDSON ALCIDES; MARIANO, FERNANDA VIVIANE; LEME, ADRIANA FRANCO PAES. Diagnostic and prognostic value of miRNAs on salivary gland tumors: a systematic review and meta-analysis. ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG, MAR 2021. (19/09692-9, 19/06809-2, 19/09419-0, 18/19922-9)

DE CARVALHO-SILVA, LARISSA TINO; NORMANDO, ANA GABRIELA C.; SA, JAMILE DE OLIVEIRA; DOS SANTOS, ERISON SANTANA; DE ROSSI, TATIANE; BUSSO-LOPES, ARIANE FIDELIS; DE OLIVEIRA, ANA KARINA; LEME, ADRIANA F. PAES. Extracellular vesicles in carcinoma microenvironment. BIOCHEMICAL SOCIETY TRANSACTIONS, v. N/A, p. 11-pg., 2023-04-06. (21/08034-8, 22/04490-1, 21/03319-4, 19/09692-9, 21/05965-0, 18/18496-6)

SA, JAMILE DE OLIVEIRA; TRINO, LUCIANA DANIELE; OLIVEIRA, ANA KARINA; BUSSO LOPES, ARIANE FIDELIS; GRANATO, DANIELA CAMPOS; COSTA NORMANDO, ANA GABRIELA; SANTOS, ERISON SANTANA; NEVES, LEANDRO XAVIER; CARNIELLI, CAROLINA MORETTO; PAES LEME, ADRIANA FRANCO. Proteomic approaches to assist in diagnosis and prognosis of oral cancer. EXPERT REVIEW OF PROTEOMICS, v. 18, n. 4, p. 261-284, APR 3 2021. (18/11958-4, 19/21815-9, 18/18496-6, 18/12194-8, 19/18751-9, 19/09692-9, 18/02180-0, 18/15535-0, 18/15728-3, 16/19337-3)

CRUZ, VITORIA MARIA SOUSA; PEREZ-DE-OLIVEIRA, MARIA EDUARDA; LEITE, EDER GERARDO DOS SANTOS; LOUREDO, BRENDO VINICIUS RODRIGUES; BEZERRA, HELEN KALINE FARIAS; LOPES, MARCIO AJUDARTE; VARGAS, PABLO AGUSTIN; SANTOS-SILVA, ALAN ROGER; KOWALSKI, LUIZ PAULO; PEREZ, DANYEL ELIAS DA CRUZ. Clinicopathological study and survival outcomes of sialoblastoma: A systematic review. HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, v. 45, n. 8, p. 13-pg., 2023-06-26. (22/01257-4, 19/09692-9, 21/10810-6)

PAULETTI, BIANCA A.; GRANATO, DANIELA C.; CARNIELLI, CAROLINA M.; CAMARA, GUILHERME A.; NORMANDO, ANA GABRIELA C.; TELLES, GUILHERME P.; LEME, ADRIANA F. PAES. Typic: A Practical and Robust Tool to Rank Proteotypic Peptides for Targeted Proteomics. JOURNAL OF PROTEOME RESEARCH, v. 22, n. 2, p. 7-pg., 2023-02-03. (19/09692-9, 18/02180-0, 19/14828-7, 18/18496-6, 18/15535-0, 19/18751-9)

DOS SANTOS, ERISON SANTANA; PEREZ-DE-OLIVEIRA, MARIA EDUARDA; COSTA NORMANDO, ANA GABRIELA; MONTEIRO GUEIROS, LUIZ ALCINO; ROGATTO, SILVIA REGINA; VARGAS, PABLO AGUSTIN; LOPES, MARCIO AJUDARTE; DA SILVA GUERRA, ELIETE NEVES; PAES LEME, ADRIANA FRANCO; SANTOS-SILVA, ALAN ROGER. Systemic conditions associated with increased risk to develop oral squamous cell carcinoma: Systematic review and meta-analysis. HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, v. 44, n. 12, p. 13-pg., 2022-09-16. (22/04490-1, 19/09692-9, 19/26676-7)

PAULETTI, BIANCA A. .; GRANATO, DANIELA C.; CARNIELLI, CAROLINA M.; CAMARA, GUILHERME A.; NORMANDO, ANA GABRIELA C.; TELLES, GUILHERME P.; LEME, ADRIANA F. PAES. Typic: A Practical and Robust Tool to Rank Proteotypic Peptides for Targeted Proteomics. JOURNAL OF PROTEOME RESEARCH, v. N/A, p. 7-pg., 2022-12-08. (19/18751-9, 19/09692-9, 18/18496-6, 18/15535-0, 19/14828-7)

DOS SANTOS, ERISON SANTANA; NORMANDO, ANA GABRIELA COSTA; SCARINI, JOAO FIGUEIRA; CRESCENCIO, LIVIA RAMALHO; DE LIMA-SOUZA, REYDSON ALCIDES; MARIANO, FERNANDA VIVIANE; LEME, ADRIANA FRANCO PAES. Diagnostic and prognostic value of miRNAs on salivary gland tumors: a systematic review and meta-analysis. ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG, v. 25, n. 4, p. 12-pg., 2021-03-05. (18/19922-9, 19/09692-9, 19/09419-0, 19/06809-2)

NORMANDO, ANA GABRIELA COSTA; DOS SANTOS, ERISON SANTANA; SA, JAMILE DE OLIVEIRA; BUSSO-LOPES, ARIANE FIDELIS; DE ROSSI, TATIANE; PATRONI, FABIO MALTA DE SA; GRANATO, DANIELA CAMPOS; GUERRA, ELIETE NEVES SILVA; SANTOS-SILVA, ALAN ROGER; LOPES, MARCIO AJUDARTE; et al. A meta-analysis reveals the protein profile associated with malignant transformation of oral leukoplakia. FRONTIERS IN ORAL HEALTH, v. 4, p. 15-pg., 2023-02-27. (22/04490-1, 19/18751-9, 21/03319-4, 19/09692-9, 18/15535-0, 21/05965-0, 18/18496-6)

NORMANDO, ANA GABRIELA COSTA; PEREZ-DE-OLIVEIRA, MARIA EDUARDA; SILVA GUERRA, ELIETE NEVES; LOPES, MARCIO AJUDARTE; ROCHA, ANDRE CAROLI; BRANDAO, THAIS BIANCA; PRADO-RIBEIRO, ANA CAROLINA; MONTEIRO GUEIROS, LUIZ ALCINO; EPSTEIN, JOEL B.; MIGLIORATI, CESAR AUGUSTO; et al. To extract or not extract teeth prior to head and neck radiotherapy? A systematic review and meta-analysis. SUPPORTIVE CARE IN CANCER, v. N/A, p. 15-pg., 2022-06-17. (19/09692-9, 19/26676-7, 18/02233-6)