Metabolic impact on the regulation of the L-arginine transporter in the polyamines pathway of Leishmania amazonensis

• Grant number: 21/04422-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2021
• End date: August 31, 2022
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: Lucile Maria Floeter-Winter
• Grantee: Juliana Ide Aoki
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/23512-0 - The Leishmania-host relationship from the ‘omics’ perspective, AP.TEM

Abstract

Leishmania is auxotrophic for amino acids and nutrients and has a specific transport for L-arginine mediated by amino acid porter-3 (AAP3) inpromastigote and amastigote forms. This transporter is encoded in two copies in the parasite's genome, one of which is regulated by the presence of the amino acid itself, externally or internally, in addition to pH, temperature and parasite arginase enzyme activity. The PD proposal has as its central objective to characterize the physiological role and cell location of the L-arginine transporter AAP3 through the application of CRISPR/Cas9 gene editing technology. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORTAZZO DA SILVA, LEONARDO; AOKI, JULIANA IDE; FLOETER-WINTER, LUCILE MARIA. Finding Correlations Between mRNA and Protein Levels in Leishmania Development: Is There a Discrepancy?. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 12, p. 16-pg., 2022-07-12. (17/23696-1, 18/23512-0, 21/04422-3)