Effects of herbicides dicamba and glyphosate isolated and combined on bees Melipona scutellaris Latreille, 1811

• Grant number: 21/04577-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2021
• Effective date (End): August 31, 2022
• Field of knowledge: Agronomical Sciences - Forestry Resources and Forestry Engineering - Nature Conservation
• Principal Investigator: Roberta Cornélio Ferreira Nocelli
• Grantee: Lucas Teodoro Lopes
• Host Institution: Centro de Ciências Agrárias (CCA). Universidade Federal de São Carlos (UFSCAR). Araras , SP, Brazil
• Associated research grant: 17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use, AP.BTA.TEM

Abstract

Native bees are key pollinators for native and cultivated plants. Understanding the effects of the products used in crops on bees is crucial and can help to establish management measures that offer more protection. The objective of this study will be to evaluate the influence of different doses of the dicamba herbicides (1 / 8D, 1 / 4D, 1 / 2D, 1D, where D = the commercial dose of 480 g / L ea / ha-1), glyphosate-salt potassium (1 / 8D, 1 / 4D, 1 / 2D, 1D, where D = the commercial dose of 480 g / L ea / ha-1) and the combination of the two in a single syrup, at a dosage of 1.0 L / ha-1 of each product, simulating its application in resistant soy according to the commercial instructions, for the survival of bees Melipona scutellaris Latreille, 1811. Its exposures will be, acute and topical, representing the contact of a forage bee in the field; and chronic oral exposure, simulating intoxication inside the hive, where the consumption of pollen and contaminated nectar can occur, observing for 96 hours. After the data collected, we will determine the doses referring to NOAEL (No Observed Adverse Effect Level) and LOAEL (Lowest Observed Adverse Effect Level). With the results obtained, we will observe the practical impacts that these herbicides cause according to the concepts of average lethal time (TL50), impact on food consumption and later a cognitive assessment with two different assessment methods, the first being an activity assessment locomotive through the displacement of the bees in a vertical direction to a fluorescent lamp, due to the phototaxis of the bees, and the second method will be the Proboscis Extension Reflex (REP), where we will observe the attraction and recognition of the food source by the bees after the intoxication. (AU)