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Effect of proanthocyanidin alone and associated with vitamin E and palm oil in preventing enamelerosion

Grant number: 21/05142-4
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2022
Effective date (End): September 30, 2023
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal Investigator:Daniela Rios Honório
Grantee:Daiana da Silva Martins
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


This in vitro study will evaluate the effectiveness of Proanthocyanidin, Palm Oil and oily Vitamin E isolated and associated in preventing initial erosion (subproject 1) and erosive tooth wear (subproject 2). Bovine teeth will be used which will be divided into 14 groups (n = 14): G1- Elmex erosion protection (positive control), G2- Deionized water (negative control), G3- 100% palm oil, G4- 6.5% Proanthocyanidin, G5- 2% Proanthocyanidin, G6- 100% Oily Vitamin E, G7- 100% Palm Oil + 6.5% Proanthocyanidin, G8- 6.5% Proanthocyanidin + Oil 100% palm oil, G9- 100% palm oil + 2% Proanthocyanidin, G10- 2% Proanthocyanidin + 100% Palm Oil, G11-Vitamin E + 6.5% Proanthocyanidin, G12 - 6.5% Proanthocyanidin + 100% Oily Vitamin E, G13- 100% Oily Vitamin E + 2% Proanthocyanidin, G14- 2% Proanthocyanidin + 100% Oily Vitamin E. The acquired pellicle will be formed in situ by using a palatal intraoral appliance, containing the enamel blocks. The treatment will be carried out by applying the solutions for 30 seconds. Soon after, the enamel blocks will be submitted to demineralization by citric acid (0.5%, pH 2.5) for 30 seconds. The response variable will be the percentage of surface hardness loss. In addition, scanning electron microscopy (SEM) will be performed for qualitative analysis of the enamel surface.. In subproject 2, only the associations that present the best results will be tested, as well as the positive and negative control groups. The acquired pellicle will be formed in situ by the use of a palatal intraoral device, containing the enamel blocks. Then, the blocks will be subjected to an erosive cycling model associated with abrasion (simulated brushing) with two treatments per day for five days. The erosive challenge will be performed with 0.1% citric acid (pH = 2.5), four times a day (90 sec). The enamel loss will be analyzed after the end of the cycling, by contact profilometry. (AU)

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