Evaluation of the retention process of bile acid by cell surface of inactivated Lacticaseibacillus rhamnosus GG via spray drying under in vitro digestion simulation

• Grant number: 23/13146-5
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: February 02, 2024
• End date: February 01, 2025
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Anderson de Souza Sant'Ana
• Grantee: Douglas Xavier dos Santos
• Supervisor: Peter Eisner
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Institution abroad: Fraunhofer-Gesellschaft, Germany
• Associated to the scholarship: 22/00190-3 - Evaluation of the postbiotic potential of inactivated Lacticaseibacillus rhamnosus GG via spray drying on the in vitro anti-inflammatory effects and its impact in obese Wistar rats, BP.PD

Abstract

Obesity is a metabolic disease featured by low-grade systemic inflammation, influenced by the interaction of several factors as lifestyle, inadequate eating habits, genetic factors, and obesogenic environment. The thermal denaturation of proteins, as well as their partial degradation, promotes an increase of the cell surface hydrophobicity of microorganisms inactivated by process of spray drying. The postbiotics (inactivated microbial cells) might promote biological activities, such as reduction cholesterol levels, modulation of the intestinal microbiota, immunomodulation, reduction of bacterial translocation, and preservation of the intestinal barrier; which effects might vary according to the probiotic strain, intensity and method of inactivation applied. In this research project, the aim is to evaluate the process of retention of bile acid by cell surface of inactivated Lacticaseibacillus rhamnosus GG via spray drying under simulating conditions of the small intestine. The use of methods based on in vitro digestion, centrifugation and dialysis, followed by quantification through high performance liquid chromatography (HPLC) will be employed to investigate how postbiotic may aýect the release of primary and secondary bile acids in relation to adsorption. As the main bile acids abundant in humans interactions with glyco- and tauroconjugated cholic acid, chenodesoxycholic acid, and desoxycholic acid will be analyzed. It is expected that the results obtained by this project provide robust scientific evidence on the possible benefits of this probiotic strain inactivated via spray drying on the obesity. By elucidating of the mechanisms of beneficial effects related to ingestion of postbiotics, it will be possible to develop a skimmed postbiotic milk powder; an innovative patentable product with several advantages in relation to probiotics. Thus, it will be possible to collaborate to the expansion of the concept of use of bacteria beneficial to human health.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

YOLMEH, MAHMOUD; XAVIER-SANTOS, DOUGLAS; SANT' ANA, ANDERSON S.. Modulating gut microbiota by paraprobiotics: Mechanisms, advantages, and challenges. FOOD BIOSCIENCE, v. 60, p. 11-pg., 2024-05-09. (21/07182-3, 22/00190-3, 23/13146-5)