Optimization of nuclear magnetic resonance spectral acquisition methods for metabolomic analysis of bovine serum: process validation

• Grant number: 23/11605-2
• Support Opportunities: Scholarships in Brazil - Support Program for Fixating Young Doctors
• Start date: September 01, 2023
• End date: February 28, 2026
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Agreement: CNPq
• Principal Investigator: Nara Regina Brandão Cônsolo
• Grantee: Fernanda Maria Marins Ocampos
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated research grant: 23/01189-1 - Optimization of nuclear magnetic resonance spectral acquisition methods for metabolomic analysis of bovine serum: process validation, AP.R

Abstract

Metabolomic analysis is a valuable tool for observing the phenotype of organisms and has been widely used in the optimization of animal production processes. One of the main techniques used in metabolomics studies is Nuclear Magnetic Resonance (NMR) spectroscopy. The main obstacle of the methodology is the intense overlapping of signals in the spectra since biological samples contain many macromolecules that prevent the observation of the metabolites of interest. The most used analysis protocol consists in removing these molecules during sample preparation using ultrafilters. Although effective, this technique is laborious and expensive due to the high unit cost of the filters. An alternative to this protocol is using NMR techniques capable of doing this removal either by manipulating molecular spins or by post-processing the spectra. However, some of these approaches, such as the CPMG pulse sequence, can interfere with the signal area, which makes it impossible to quantify the metabolites. This project focuses on validating two selected methodologies (DREAMTIME and FDM) that have the potential to replace the sample filtration process. This proposal is linked to the FAPESP process 2020/08845-3 since the same samples will be used for both projects. The results of this project may bring greater agility and efficiency in processing samples and obtaining biofluid spectra, eliminating the need for ultrafilters without prejudice to the quantification of metabolites.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RAMOS, PATRICIA MALOSO; DELGADO, EDUARDO FRANCISQUINE; DA SILVA, ANA CLAUDIA; CONSOLO, NARA REGINA BRANDAO; HERREIRA, VINICIUS LAERTE SILVA; VALIM, JOAO MARCOS BOVETTO DE CAMPOS; OCAMPOS, FERNANDA MARIA MARINS; COLNAGO, LUIZ ALBERTO; DA LUZ E SILVA, SAULO. Temperament Upregulates Mitochondrial Enzymes and Negatively Affects Myofibrillar Fragmentation in Beef of Excitable Bos taurus indicus Cattle. METABOLITES, v. 15, n. 1, p. 19-pg., 2025-01-01. (21/10205-5, 23/11605-2)