Application of water kefir in co-fermentation with Saccharomyces boulardii for the development of probiotic mead (physicochemical, microbiological, sensory characteristics, in vitro digestibility and effect on metabolic profile in an in vivo model)

• Grant number: 22/12187-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2024
• Status: Discontinued
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Eliana Setsuko Kamimura
• Grantee: Handray Fernandes de Souza
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated scholarship(s): 24/19375-9 - Influence of low-frequency ultrasound on Saccharomyces cerevisiae var. boulardii: Effects on mead fermentation and bioactive properties, BE.EP.DR

Abstract

Mead is a beverage containing 4% to 14% (v/v) of ethanol, produced by alcoholic fermentation of a diluted honey solution and the action of yeast. In mead production the most commonly used strain is Saccharomyces cerevisiae (S. cerevisiae). However, the use of non-conventional microorganisms is potentially promising, in which Saccharomyces boulardii (S. boulardii) and water kefir stand out. According to the literature, studies have already evaluated the potential of S. boulardii in the development of potentially probiotic mead and kefir in the development of probiotic fermented alcoholic beverages such as craft beers. However, there are no reports of co-fermentation of water kefir and S. boulardii for mead development. In this study, mead formulations using S. boulardii and water kefir as starter cultures in co-fermentation; fermentation kinetics; physicochemical characteristics of the product; viable cell counts; shelf life of the mead; survival of the microorganisms to simulated gastric and intestinal juices in vitro; the effect on the metabolic profile in an in vivo mouse model; and sensory analysis of the mead will be studied. In this context, this research seeks to evaluate the potential of S. boulardii and water kefir for the development of probiotic mead.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, HANDRAY FERNANDES; FREIRE, EDUARDO NOVAIS SOUZA; MONTEIRO, GIOVANA FELICIO; BOGAZ, LORENA TEIXEIRA; TEIXEIRA, RICARDO DONIZETE; SILVA JUNIOR, FABIANO VAQUERO; TEIXEIRA, FELIPE DONIZETE; DOS SANTOS, JOAO VITOR; DE CARVALHO, MARINA VIEIRA; ROCHA, RAMON DA SILVA; et al. Development of Potentially Probiotic Mead from Co-Fermentation by Saccharomyces cerevisiae var. boulardii and Kombucha Microorganisms. FERMENTATION-BASEL, v. 10, n. 9, p. 15-pg., 2024-09-01. (22/12187-7, 23/18012-7)

PEREIRA, KARINA NASCIMENTO; DE SOUZA, HANDRAY FERNANDES; DE OLIVEIRA, AMANDA CRISTINA DIAS; DEZIDERIO, MARCELA APARECIDA; DE CARVALHO, MARINA VIEIRA; ROCHA, RAMON S.; CRUZ, ADRIANO G.; DE OLIVEIRA, CARLOS AUGUSTO FERNANDES; KAMIMURA, ELIANA SETSUKO. Technological aspects of Saccharomyces cerevisiae var. boulardii applications in fermented alcoholic beverages: A review. QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS, v. 17, n. 3, p. 16-pg., 2025-01-01. (22/12187-7)