Tissue response to implanted infected dentine tubes irrigated with 23ppm silver nanoparticles dispersion or 2,5% sodium hypochlorite.

• Grant number: 09/16068-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2010
• End date: December 31, 2010
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: João Eduardo Gomes Filho
• Grantee: Karina Vanessa Tendoro
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The aim of this study was to evaluate the antimicrobial effect of silver nanoparticles dispersion using the tissue response to implanted dentine tubes irrigated with silver nanoparticles dispersion or 2.5% sodium hyppochlorite. Forty rats will individually receive two dentine tubes irrigated with 47ppm silver nanoparticles dispersion, 2.5% sodium hypochlorite, saline or sterilized tube as control. The observation periods will be 7 and 30 days. After each period of time, twenty animals will be killed and the tubes and surrounding tissue removed, fixed and prepared to be analyzed in light microscope with glycol methacrylate embedding, 3 mm serial cutting and HE staining. Scores will be defined as follows: 0, none or few inflammatory cells, no reaction; 1, <25 cells, mild reaction; 2, 25 to 125 cells, moderate reaction; 3, >125 cells, severe reaction. Fibrous capsule was categorized as "thin" when thickness was < 150 ¼m and "thick" at > 150 ¼m. Necrosis and formation of calcification were recorded. The data will be statistically analyzed using Kruskal Wallis test.