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Influence of the fluoride in phosphatase and matrix metalloproteinase 2 and 9 activity, in osteoblasts.

Grant number: 08/00322-0
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2008
Effective date (End): April 30, 2009
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Rodrigo Cardoso de Oliveira
Grantee:Camila Slompo
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


The aim of the present project will be to evaluate the behavior of human osteoblasts submitted the different fluoride concentration. Cells hFOB 1.19 will be incubated with different fluoride concentration (5 µM, 10 µM, 100 µM e 1 mM) at 37oC, at 24, 48, 72, 96 and 120 hour. The conditional and osteoblasts ways will be collected and evaluated how much to the cellular viability (counting of cells, reduction of the MTT and Neutral Red) and activity of enzymes (total acid phosphatase (FAT), lisossomal phosphatase (FAL) and phosphotyrosine phosphatase (PTP)). After the preparation of proteins of the human osteoblasts, the same ones will be submitted to zymografia. The gels of the analyzed groups have controlled and treat they will be scanned using the ImageScanner and with software ImageMaster Platinum. The statistical analyses will be made through the tests ANOVA, and have tested "t", in accordance with its indication (Tukey`s test if p<0.05).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CAMILA SLOMPO; CAMILA P. BUZALAF; CARLA A. DAMANTE; GISELE M. MARTINS; ANGÉLICA R HANNAS; MARÍLIA A. R BUZALAF; RODRIGO C OLIVEIRA. Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities. Brazilian Dental Journal, v. 23, n. 6, p. 629-634, . (08/00322-0)

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