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| Author(s): |
Eloísa Berbel Manaia
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Araraquara. 2016-06-30. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara |
| Defense date: | 2016-05-25 |
| Advisor: | Leila Aparecida Chiavacci; Claudie Bourgaux |
| Abstract | |
Theranostic systems consist of a single device containing therapeutics and diagnosis agents and have increased attention in the actual researches because these devices can improve the disease therapy such as cancer, decrease the side effects and the toxicity in no-cancer cells and permit monitoring the treatment. The aim of this work was to develop theranostic systems consisted of lipid based nanocarriers containing ZnO based quantum dots (QDs) as cancer cell luminescent guides, and a model drug for cancer therapy. Firstly, it was study the synthesis of ZnO/ZnS QDs aiming to achieve improved luminescent properties. In this step, X-Rays Absorption Spectroscopy, together with other usual characterization techniques, could identify the synthesis condition in which core-shell structures were formed. Nevertheless, the emission of ZnO/ZnS QDs in the visible range was not promising. Therefore, Mg-doped ZnO QDs were synthesized and their luminescence went through a maximum for a 20 mol% nominal concentration of Mg2+ ions in the reaction medium. Zn0.8Mg0.2O QDs presented quantum yield (QY) six times higher (QY = 64%) than undoped ones (QY = 10%). ZnO and Zn0.8Mg0.2O QDs capped by oleic acid (OA) were synthesized and formed stable colloidal dispersions in chloroform and toluene. The QY of OA-Zn0.8Mg0.2O was about 4 times (around 40%) higher than that of the OA-ZnO QDs. Zn0.8Mg0.2O QDs and OA-Zn0.8Mg0.2O QDs could be incorporated into lipid based nanocarriers of average hydrodynamic diameter around 100 – 220 nm. The luminescent solid lipid nanoparticles (SLN) were stable in different media at 37°C during 3 hours. The fluorescence association study showed enhanced emission of the J774 macrophage-like cells treated with 2 mg/mL of luminescent SLN during 50 min, suggesting partial internalization of the nanoparticles into the macrophages. The internalization study using the video-microscope and fluorescence microscope were not successfully, once the equipment condition used could not overcome the cells auto-fluorescence phenomena. (AU) | |
| FAPESP's process: | 12/07570-4 - Zinc based quantum dots and double salts incorporated in liposome for image and vectorization of anticancer drug |
| Grantee: | Eloísa Berbel Manaia |
| Support Opportunities: | Scholarships in Brazil - Doctorate |