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| Author(s): |
Daniel de Souza Ramos Angrimani
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2018-02-08 |
| Examining board members: |
Camila Infantosi Vannucchi;
Maria Denise Lopes;
Marcilio Nichi;
Fabiana Ferreira de Souza;
Gisele Almeida Lima da Veiga
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| Advisor: | Camila Infantosi Vannucchi |
| Abstract | |
Benign prostatic hyperplasia (BPH) is the disease of highest incidence in senile dogs. Thus, it is essential to characterize the pathogenesis of the disease, including the evaluation of animals submitted to clinical or surgical treatment (finasteride therapy and orchiectomy). In this context, the aim of this study was to characterize BPH changes of dogs submitted to different treatments. Therefore, a total of 25 dogs were used (BPH and healthy), applying distinct experimental tools: Doppler ultrasonography to characterize prostatic and testicular hemodynamic changes involved, immunohistochemistry and PCR for the expression of prostatic VEGF factor, testicular morphohistologic analysis, hormonal assay (testosterone, estrogen and dihydrotestosterone), breeding soundness examination, seminal analysis and oxidative stress and antioxidant enzymes evaluation of the prostatic fluid. It was possible to observe a reduction in prostatic vascularization score (by Color Doppler) in finasteride treated and orchiectomized animals, with an additional reduction of the prostatic volume. Finasteride treatment reduced VEGF expression by immunohistochemistry, however, orchiectomy also reduced VEGF gene expression. In addition, finasteride treatment reduced dihydrotestosterone concentration, without significant impact to seminal quality. On the other hand, BPH promoted significant changes in testosterone concentration and seminal quality, such as poor sperm concentration, changes in the sperm kinetics and high rates of sperm DNA fragmentation. In conclusion, prostatic VEGF activity in dogs is dependent on the occurrence of BPH and different therapies, promoting also differences in prostatic vascularization and volume. Furthermore, dogs with BPH had hormonal and spermatogenesis changes, however, the short course of finasteride treatment (2 months) was able to favor the disease overcome, favoring the application of new approaches to HPB diagnosis and prognosis in dogs. (AU) | |
| FAPESP's process: | 13/25966-5 - Characterization of seminal and hemodynamic changes in the prostate and testes of dogs with benign prostatic hyperplasia |
| Grantee: | Daniel de Souza Ramos Angrimani |
| Support Opportunities: | Scholarships in Brazil - Doctorate |